Hydrostatic pressure reduces thrombogenicity of polytetrafluoroethylene vascular grafts

Edmond F Ritter, R. D. Vann, C. Wyble, W. J. Barwick, B. Klitzman

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A prime factor in the thrombogenicity of synthetic materials in contact with blood is the blood-gas interface. Small pockets of gas, known as gas nuclei, are trapped within surface interstices. The resulting blood-gas interface denatures plasma proteins and activates clotting factors and platelets. Expanded polytetrafluoroethylene (ePTFE) vascular prostheses 1 mm in internal diameter were placed in saline under 6,000 psig hydrostatic pressure for 2 h in an attempt to dissolve all gas nuclei (i.e., to denucleate). Carotid-carotid bypasses were performed in rats using 280-mm lengths of ePTFE. All 10 control grafts lost patency in 5 min, whereas the 14 denucleated grafts had a median patency duration of 300 min (P < 0.01). In 15-mm-long rat femoral artery interpositional ePTFE grafts, 90% of controls thrombosed within 10 min, whereas only 7% of denucleated grafts thrombosed over the duration of the 7-day observation period (P < 0.001). Denucleation also resulted in a significant reduction (P < 0.02) in 111In-labeled platelet adhesion to 36% of control. Scanning electron microscopy confirmed the reduced accumulation of platelets on denucleated grafts. These data suggest that the removal of trapped air with hydrostatic pressure significantly reduces the thrombogenicity of ePTFE microvascular prostheses and may have application to other clinical (catheters, valves, tubing, etc.) or experimental (micropipettes, electrodes, etc.) materials that interface with blood.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume257
Issue number4
StatePublished - Jan 1 1989

Fingerprint

Hydrostatic Pressure
Polytetrafluoroethylene
Blood Vessels
Gases
Transplants
Blood Platelets
Thrombosis
Blood Vessel Prosthesis
Blood Coagulation Factors
Femoral Artery
Electron Scanning Microscopy
Prostheses and Implants
Blood Proteins
Electrodes
Catheters
Air
Observation

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Hydrostatic pressure reduces thrombogenicity of polytetrafluoroethylene vascular grafts. / Ritter, Edmond F; Vann, R. D.; Wyble, C.; Barwick, W. J.; Klitzman, B.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 257, No. 4, 01.01.1989.

Research output: Contribution to journalArticle

@article{e2ba8e7168624e47a3e59467a2ac8214,
title = "Hydrostatic pressure reduces thrombogenicity of polytetrafluoroethylene vascular grafts",
abstract = "A prime factor in the thrombogenicity of synthetic materials in contact with blood is the blood-gas interface. Small pockets of gas, known as gas nuclei, are trapped within surface interstices. The resulting blood-gas interface denatures plasma proteins and activates clotting factors and platelets. Expanded polytetrafluoroethylene (ePTFE) vascular prostheses 1 mm in internal diameter were placed in saline under 6,000 psig hydrostatic pressure for 2 h in an attempt to dissolve all gas nuclei (i.e., to denucleate). Carotid-carotid bypasses were performed in rats using 280-mm lengths of ePTFE. All 10 control grafts lost patency in 5 min, whereas the 14 denucleated grafts had a median patency duration of 300 min (P < 0.01). In 15-mm-long rat femoral artery interpositional ePTFE grafts, 90{\%} of controls thrombosed within 10 min, whereas only 7{\%} of denucleated grafts thrombosed over the duration of the 7-day observation period (P < 0.001). Denucleation also resulted in a significant reduction (P < 0.02) in 111In-labeled platelet adhesion to 36{\%} of control. Scanning electron microscopy confirmed the reduced accumulation of platelets on denucleated grafts. These data suggest that the removal of trapped air with hydrostatic pressure significantly reduces the thrombogenicity of ePTFE microvascular prostheses and may have application to other clinical (catheters, valves, tubing, etc.) or experimental (micropipettes, electrodes, etc.) materials that interface with blood.",
author = "Ritter, {Edmond F} and Vann, {R. D.} and C. Wyble and Barwick, {W. J.} and B. Klitzman",
year = "1989",
month = "1",
day = "1",
language = "English (US)",
volume = "257",
journal = "American Journal of Physiology - Heart and Circulatory Physiology",
issn = "0363-6135",
publisher = "American Physiological Society",
number = "4",

}

TY - JOUR

T1 - Hydrostatic pressure reduces thrombogenicity of polytetrafluoroethylene vascular grafts

AU - Ritter, Edmond F

AU - Vann, R. D.

AU - Wyble, C.

AU - Barwick, W. J.

AU - Klitzman, B.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - A prime factor in the thrombogenicity of synthetic materials in contact with blood is the blood-gas interface. Small pockets of gas, known as gas nuclei, are trapped within surface interstices. The resulting blood-gas interface denatures plasma proteins and activates clotting factors and platelets. Expanded polytetrafluoroethylene (ePTFE) vascular prostheses 1 mm in internal diameter were placed in saline under 6,000 psig hydrostatic pressure for 2 h in an attempt to dissolve all gas nuclei (i.e., to denucleate). Carotid-carotid bypasses were performed in rats using 280-mm lengths of ePTFE. All 10 control grafts lost patency in 5 min, whereas the 14 denucleated grafts had a median patency duration of 300 min (P < 0.01). In 15-mm-long rat femoral artery interpositional ePTFE grafts, 90% of controls thrombosed within 10 min, whereas only 7% of denucleated grafts thrombosed over the duration of the 7-day observation period (P < 0.001). Denucleation also resulted in a significant reduction (P < 0.02) in 111In-labeled platelet adhesion to 36% of control. Scanning electron microscopy confirmed the reduced accumulation of platelets on denucleated grafts. These data suggest that the removal of trapped air with hydrostatic pressure significantly reduces the thrombogenicity of ePTFE microvascular prostheses and may have application to other clinical (catheters, valves, tubing, etc.) or experimental (micropipettes, electrodes, etc.) materials that interface with blood.

AB - A prime factor in the thrombogenicity of synthetic materials in contact with blood is the blood-gas interface. Small pockets of gas, known as gas nuclei, are trapped within surface interstices. The resulting blood-gas interface denatures plasma proteins and activates clotting factors and platelets. Expanded polytetrafluoroethylene (ePTFE) vascular prostheses 1 mm in internal diameter were placed in saline under 6,000 psig hydrostatic pressure for 2 h in an attempt to dissolve all gas nuclei (i.e., to denucleate). Carotid-carotid bypasses were performed in rats using 280-mm lengths of ePTFE. All 10 control grafts lost patency in 5 min, whereas the 14 denucleated grafts had a median patency duration of 300 min (P < 0.01). In 15-mm-long rat femoral artery interpositional ePTFE grafts, 90% of controls thrombosed within 10 min, whereas only 7% of denucleated grafts thrombosed over the duration of the 7-day observation period (P < 0.001). Denucleation also resulted in a significant reduction (P < 0.02) in 111In-labeled platelet adhesion to 36% of control. Scanning electron microscopy confirmed the reduced accumulation of platelets on denucleated grafts. These data suggest that the removal of trapped air with hydrostatic pressure significantly reduces the thrombogenicity of ePTFE microvascular prostheses and may have application to other clinical (catheters, valves, tubing, etc.) or experimental (micropipettes, electrodes, etc.) materials that interface with blood.

UR - http://www.scopus.com/inward/record.url?scp=0024451262&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024451262&partnerID=8YFLogxK

M3 - Article

C2 - 2801970

AN - SCOPUS:0024451262

VL - 257

JO - American Journal of Physiology - Heart and Circulatory Physiology

JF - American Journal of Physiology - Heart and Circulatory Physiology

SN - 0363-6135

IS - 4

ER -