Hypertension in L-NAME-treated diabetic rats depends on an intact sympathetic nervous system

Sharyn M. Fitzgerald, Michael W. Brands

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

We demonstrated previously that induction of diabetes in rats that were treated chronically with the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) causes a severe, progressive increase in mean arterial pressure. This study tested the role of the sympathetic nervous system in that response. Rats were instrumented with chronic artery and vein catheters and assigned randomly to four diabetic groups pretreated with vehicle (D), L-NAME (D+L), the α1- and β-adrenergic receptor antagonists terazosin and propranolol (D+B), or L-NAME, terazosin, and propranolol (D+LB). After baseline measurements were taken, rats were pretreated; 6 days later, streptozotocin was administered and 3 wk of diabetes ensued. D+L rats had a marked, progressive increase in arterial pressure that by day 20 was ∼60 mmHg greater than in D rats. The pressor response to L-NAME was significantly attenuated in diabetic rats cotreated with adrenergic blockers. During week 1 of diabetes, plasma renin activity (PRA) increased and then returned to control levels in D rats. PRA increased progressively in D+L rats, and chronic adrenergic receptor blockade restored the biphasic renin response in D+LB rats. These results suggest that the sympathetic nervous system may be involved in the hypertensive response to onset of diabetes in L-NAME-treated rats, possibly through control of renin secretion.

Original languageEnglish (US)
Pages (from-to)R1070-R1076
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume282
Issue number4 51-4
DOIs
StatePublished - 2002

Keywords

  • Angiotensin II
  • Glomerular filtration rate
  • Glucose
  • Mean arterial pressure
  • N-nitro-L-arginine methyl ester
  • Nitric oxide

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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