Hypotheses regarding the role of pericytes in regulating movement of fluid, nutrients, and hormones across the microcirculatory endothelial barrier

A. J. Lonigro, L. McMurdo, A. H. Stephenson, R. S. Sprague, Neal Lee Weintraub

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A decade ago, we initiated studies to define relationship(s) between products of 5-lipoxygenase-mediated arachidonic acid metabolism and altered microvascular permeability. Patients with permeability (nonhydrostatic) pulmonary edema (adult respiratory distress syndrome) and intact animal models of permeability edema, produced with agents that required neutrophils (phorbol myristate acetate) and those that did not (ethchlorvynol), invariably revealed the presence of leukotrienes; in contrast, leukotrienes were not detected in cases of hydrostatic pulmonary edema. In isolated perfused canine lung, we identified increases in microvascular permeability coefficients in response to the injurious agent. Permeability coefficients were not increased when injurinous agents were given in the presence of 5- lipoxygenase inhibitors. To define further the relationships between leukotriene generation and edema formation, we postulated that leukotrienes effected contraction of capillary pericytes, thereby increasing pore size of endothelial intercellular junctions and enhancing movement across the microvascular barrier. We isolated pericytes from bovine retinas, identified them morphologically and by staining characteristics, and, in preliminary experiments, found that they do not possess the 5-lipoxygenase enzyme; however, when cocultured with neutrophils, which possess 5-lipoxygenase but cannot synthesize sulfidopeptide leukotrienes because of their lack of glutathione S-transferase, sulfidopeptide leukotriene synthesis ensued. In view of the anatomic position of pericytes, evidence that they participate in endothelial transport, their ability to contract, and evidence of cell-to- cell communication, we propose that pericytes control the movement of fluid, solutes, hormones, and small and large molecules across the microvascular endothelium.

Original languageEnglish (US)
JournalDiabetes
Volume45
Issue number1 SUPPL.
StatePublished - Jan 8 1996
Externally publishedYes

Fingerprint

Pericytes
Leukotrienes
Hormones
Arachidonate 5-Lipoxygenase
Food
Permeability
Capillary Permeability
Pulmonary Edema
Ethchlorvynol
Edema
Neutrophils
Lipoxygenase Inhibitors
Intercellular Junctions
Adult Respiratory Distress Syndrome
Tetradecanoylphorbol Acetate
Glutathione Transferase
Cell Communication
Endothelium
Retina
Canidae

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Hypotheses regarding the role of pericytes in regulating movement of fluid, nutrients, and hormones across the microcirculatory endothelial barrier. / Lonigro, A. J.; McMurdo, L.; Stephenson, A. H.; Sprague, R. S.; Weintraub, Neal Lee.

In: Diabetes, Vol. 45, No. 1 SUPPL., 08.01.1996.

Research output: Contribution to journalArticle

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