Identification and Characterization of a Family of Rab11-interacting Proteins

Chadwick M. Hales, Richard D Griner, Karen C. Hobdy-Henderson, Matthew C. Dorn, David Hardy, Ravindra Kumar, Jennifer Navarre, Edward K.L. Chan, Lynne A. Lapierre, James R. Goldenring

Research output: Contribution to journalArticle

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Abstract

Rab11a is a small GTP-binding protein enriched in the pericentriolar plasma membrane recycling systems. We hypothesized that Rab11a-binding proteins exist as downstream effectors of its action. Here we define a family of four Rab11-interacting proteins: Rab11-Family Interacting Protein 1 (Rab11-FIP1), Rab11-Family Interacting Protein 2 (Rab11-FIP2), Rab11-Family Interacting Protein 3 (Rab11-FIP3), and pp75/Rip11. All four interacting proteins associated with wild type Rab11a and dominant active Rab11a (Rab11aS20V) as well as Rab11b and Rab25. Rab11-FIP2 also interacted with dominant negative Rab11a (Rab11aS25N) and the tail of myosin Vb. The binding of Rab11-FIP1, Rab11-FIP2, and Rab11-FIP3 to Rab11a was dependent upon a conserved carboxyl-terminal amphipathic α-helix. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 colocalized with Rab11a in plasma membrane recycling systems in both non-polarized HeLa cells and polarized Madin-Darby canine kidney cells. GFP-Rab11-FIP3 also colocalized with Rab11a in HeLa cells. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 also coenriched with Rablla and H+K +-ATPase on parietal cell tubulovesicles, and Rab11-FIP1 and Rab11-FIP2 translocated with Rablla and the H+K+-ATPase upon stimulating parietal cells with histamine. The results suggest that the function of Rablla in plasma membrane recycling systems is dependent upon a compendium of protein effectors.

Original languageEnglish (US)
Pages (from-to)39067-39075
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number42
DOIs
StatePublished - Oct 19 2001

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Proteins
Proton-Translocating ATPases
Cell Membrane
HeLa Cells
Cell membranes
Recycling
rab11 protein
Madin Darby Canine Kidney Cells
Myosins
Adenosine Triphosphatases
GTP-Binding Proteins
Histamine
Carrier Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hales, C. M., Griner, R. D., Hobdy-Henderson, K. C., Dorn, M. C., Hardy, D., Kumar, R., ... Goldenring, J. R. (2001). Identification and Characterization of a Family of Rab11-interacting Proteins. Journal of Biological Chemistry, 276(42), 39067-39075. https://doi.org/10.1074/jbc.M104831200

Identification and Characterization of a Family of Rab11-interacting Proteins. / Hales, Chadwick M.; Griner, Richard D; Hobdy-Henderson, Karen C.; Dorn, Matthew C.; Hardy, David; Kumar, Ravindra; Navarre, Jennifer; Chan, Edward K.L.; Lapierre, Lynne A.; Goldenring, James R.

In: Journal of Biological Chemistry, Vol. 276, No. 42, 19.10.2001, p. 39067-39075.

Research output: Contribution to journalArticle

Hales, CM, Griner, RD, Hobdy-Henderson, KC, Dorn, MC, Hardy, D, Kumar, R, Navarre, J, Chan, EKL, Lapierre, LA & Goldenring, JR 2001, 'Identification and Characterization of a Family of Rab11-interacting Proteins', Journal of Biological Chemistry, vol. 276, no. 42, pp. 39067-39075. https://doi.org/10.1074/jbc.M104831200
Hales, Chadwick M. ; Griner, Richard D ; Hobdy-Henderson, Karen C. ; Dorn, Matthew C. ; Hardy, David ; Kumar, Ravindra ; Navarre, Jennifer ; Chan, Edward K.L. ; Lapierre, Lynne A. ; Goldenring, James R. / Identification and Characterization of a Family of Rab11-interacting Proteins. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 42. pp. 39067-39075.
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AU - Hardy, David

AU - Kumar, Ravindra

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