Identification and Characterization of a Family of Rab11-interacting Proteins

Chadwick M. Hales, Richard Griner, Karen C. Hobdy-Henderson, Matthew C. Dorn, David Hardy, Ravindra Kumar, Jennifer Navarre, Edward K.L. Chan, Lynne A. Lapierre, James R. Goldenring

Research output: Contribution to journalArticle

227 Scopus citations

Abstract

Rab11a is a small GTP-binding protein enriched in the pericentriolar plasma membrane recycling systems. We hypothesized that Rab11a-binding proteins exist as downstream effectors of its action. Here we define a family of four Rab11-interacting proteins: Rab11-Family Interacting Protein 1 (Rab11-FIP1), Rab11-Family Interacting Protein 2 (Rab11-FIP2), Rab11-Family Interacting Protein 3 (Rab11-FIP3), and pp75/Rip11. All four interacting proteins associated with wild type Rab11a and dominant active Rab11a (Rab11aS20V) as well as Rab11b and Rab25. Rab11-FIP2 also interacted with dominant negative Rab11a (Rab11aS25N) and the tail of myosin Vb. The binding of Rab11-FIP1, Rab11-FIP2, and Rab11-FIP3 to Rab11a was dependent upon a conserved carboxyl-terminal amphipathic α-helix. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 colocalized with Rab11a in plasma membrane recycling systems in both non-polarized HeLa cells and polarized Madin-Darby canine kidney cells. GFP-Rab11-FIP3 also colocalized with Rab11a in HeLa cells. Rab11-FIP1, Rab11-FIP2, and pp75/Rip11 also coenriched with Rablla and H+K +-ATPase on parietal cell tubulovesicles, and Rab11-FIP1 and Rab11-FIP2 translocated with Rablla and the H+K+-ATPase upon stimulating parietal cells with histamine. The results suggest that the function of Rablla in plasma membrane recycling systems is dependent upon a compendium of protein effectors.

Original languageEnglish (US)
Pages (from-to)39067-39075
Number of pages9
JournalJournal of Biological Chemistry
Volume276
Issue number42
DOIs
StatePublished - Oct 19 2001

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Hales, C. M., Griner, R., Hobdy-Henderson, K. C., Dorn, M. C., Hardy, D., Kumar, R., Navarre, J., Chan, E. K. L., Lapierre, L. A., & Goldenring, J. R. (2001). Identification and Characterization of a Family of Rab11-interacting Proteins. Journal of Biological Chemistry, 276(42), 39067-39075. https://doi.org/10.1074/jbc.M104831200