Identification and characterization of cytosolic sulfotransferases in normal human endometrium

Josie L. Falany, Ricardo Azziz, Charles N. Falany

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Understanding the factors which alter estrogen metabolism and activity in endometrial tissue is important because unopposed estrogen stimulation is an important risk factor in the development of endometrial carcinoma. The cyclic progression of the endometrium through proliferative and scaretory phases is normally under the control of the ovarian hormones β-estradiol (E2) and progesterone. One mechanism by which progesterone inhibits the activity of E2 in secretory endometrium is by elevating the degree of E2 sulfation, thereby reducing its ability to bind to the estrogen receptor and elicit a cellular response. Our laboratories have investigated the cytosolic sulfotransferases (STs) found in biopsies of both proliferative and secretory endometrium obtained from five normal pre-menopausal women who were not taking any drugs or steroids. Two of the human cytosolic STs were detected in human endometrial tissues. The phenol-sulfating form of phenol ST (P-PST) was found at varying levels in cytosol from both proliferative and secretory endometrium in all of the women studied but with no consistent correlation to the phase of the menstrual cycle. In contrast, estrogen ST (EST) was not detected in the proliferative endometrial cytosol of any of the women studied but was consistently found in all of the secretory endometrial cytosols. The presence and levels of these STs was confirmed by ST activity studies, immunoblot analysis and Northern blot analysis. These results indicate that the expression of EST in human endometrial tissues varies with the phase of the menstrual cycle and is most likely regulated by progesterone secreted from the ovaries.

Original languageEnglish (US)
Pages (from-to)329-339
Number of pages11
JournalChemico-Biological Interactions
Volume109
Issue number1-3
DOIs
StatePublished - Feb 20 1998

Keywords

  • Endometrium
  • Estrogen
  • Human
  • Sulfation
  • Sulfotransferase

ASJC Scopus subject areas

  • Toxicology

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