Identification and characterization of estrogen-like components in commercial resin-based dental restorative materials.

J. B. Lewis, Frederick Rueggeberg, C. A. Lapp, J. W. Ergle, G. S. Schuster

Research output: Contribution to journalArticle

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Abstract

Recently, resin-based dental restorative materials have been targeted as potential sources of xenoestrogens, specifically bisphenol A (BPA) and bisphenol A dimethacrylate (BAD), which could contribute to overall estrogen load and result in deleterious side effects. The present study used high-pressure liquid chromatography (HPLC) to analyze twenty-eight different commercially available dental resins for the presence of BPA and/or BAD. In addition, sublines of the MCF-7 human breast tumor cell line were cultured in the presence of eluates from eleven of the dental resins and assessed for proliferative responses using the sulforhodamine B assay. Only one resin, Delton II, had detectable levels of BPA or BAD that could be verified by Fourier transform infrared spectrometry. Likewise, eluates from Delton II were the only samples that elicited a significant proliferative response in two of the MCF-7 sublines tested. Therefore, we conclude that dental resins in general do not represent a significant source of BPA or BAD exposure.

Original languageEnglish (US)
Pages (from-to)107-113
Number of pages7
JournalClinical Oral Investigations
Volume3
Issue number3
DOIs
StatePublished - Jan 1 1999

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Dental Materials
Bisphenol A-Glycidyl Methacrylate
Synthetic Resins
Estrogens
lissamine rhodamine B
Fourier Analysis
Tumor Cell Line
Spectrum Analysis
High Pressure Liquid Chromatography
Breast Neoplasms
2,2-di(4-methacryloxyphenyl)propane
bisphenol A

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Identification and characterization of estrogen-like components in commercial resin-based dental restorative materials. / Lewis, J. B.; Rueggeberg, Frederick; Lapp, C. A.; Ergle, J. W.; Schuster, G. S.

In: Clinical Oral Investigations, Vol. 3, No. 3, 01.01.1999, p. 107-113.

Research output: Contribution to journalArticle

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