Identification and Localization of a Cysteinyl Residue Critical for the Trypsin-like Catalytic Activity of the Proteasome

Lawrence R. Dick, Carolyn R. Moomaw, Bikash C. Pramanik, George N. DeMartino, Clive A. Slaughter

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Chemical modification of the proteasome with 7V-ethylmaleimide (NEM) was performed for the purpose of identifying amino acid residues that play a role in the enzyme's proteolytic function. Modification of the proteasome with NEM specifically and irreversibly suppressed one of the three peptidase activities of the enzyme, viz., the“trypsin-like”activity. Leupeptin, a reversible competitive inhibitor of this activity, protected the activity from NEM inactivation, suggesting that NEM modifies a residue in the leupeptin binding site. Comparisons of enzyme samples labeled with [14C]NEM either in the presence or in the absence of leupeptin allowed the identification of a proteasome subunit containing an NEM-modified, leupeptin-protected cysteinyl residue. The leupeptin protection experiments suggest that residues of this subunit contribute to the active site responsible for the proteasome‘s trypsin-like activity. This subunit was purified by reverse-phase high-performance liquid chromatography. Peptide mapping and N-terminal amino acid sequencing were employed to acquire information about the primary structure of the subunit, including the sequence surrounding the leupeptin-protected cysteinyl residue. The sequencing data suggest that this proteasome subunit is evolutionarily related to other proteasome subunits that have been sequenced, which show no homology to other known proteases. The assignment of a catalytic function to a member of the proteasome family supports the hypothesis that proteasome subunits represent a structurally and possibly mechanistically novel group of proteases.

Original languageEnglish (US)
Pages (from-to)7347-7355
Number of pages9
JournalBiochemistry
Volume31
Issue number32
DOIs
StatePublished - Feb 1 1992

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Proteasome Endopeptidase Complex
Trypsin
Catalyst activity
Peptide Hydrolases
Amino Acids
Ethylmaleimide
Peptide Mapping
Protein Sequence Analysis
Chemical modification
High performance liquid chromatography
Reverse-Phase Chromatography
Enzymes
leupeptin
Catalytic Domain
Binding Sites
High Pressure Liquid Chromatography
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Identification and Localization of a Cysteinyl Residue Critical for the Trypsin-like Catalytic Activity of the Proteasome. / Dick, Lawrence R.; Moomaw, Carolyn R.; Pramanik, Bikash C.; DeMartino, George N.; Slaughter, Clive A.

In: Biochemistry, Vol. 31, No. 32, 01.02.1992, p. 7347-7355.

Research output: Contribution to journalArticle

Dick, Lawrence R. ; Moomaw, Carolyn R. ; Pramanik, Bikash C. ; DeMartino, George N. ; Slaughter, Clive A. / Identification and Localization of a Cysteinyl Residue Critical for the Trypsin-like Catalytic Activity of the Proteasome. In: Biochemistry. 1992 ; Vol. 31, No. 32. pp. 7347-7355.
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