Identification, cellular localization, isolation, and characterization of human Clara cell-specific 10 KD protein

Gurmukh Singh, J. Singh, S. L. Katyal, W. E. Brown, J. A. Kramps, I. L. Paradis, J. H. Dauber, T. A. Macpherson, N. Squeglia

Research output: Contribution to journalArticle

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Abstract

Human lung lavage proteins were fractionated by centrifugation and molecular sieving. An antiserum to the post-albumin fraction of the soluble proteins reacted with a 10 KD protein and this protein was isolated by conventional chromatography. The protein, which has a pI of 4.8, consists of two 5 KD polypeptides and is rich in glutamic acid, leucine, serine, and aspartic acid amino acids. The protein does not bind to concanavalin A, pancreatic elastase, leukocyte elastase, or trypsin, and lacks anti-protease activity. It constitutes about 0.15% of the soluble proteins in lung lavage. Antibodies to the 10 KD protein specifically and exclusively stain Clara cells in human, dog, and rat. Staining of granules of Clara cells was prominent in the distal bronchioles; however, the non-ciliated cells of respiratory bronchioles did not stain for the 10 KD protein. This 10 KD protein appears in fetal lungs at 21 weeks of gestation, and was present in about 10% of the primary pulmonary adenocarcinomas. As a specific marker for Clara cells, this protein could be useful in the study of development, regulation of secretion, and pathobiology of these cells.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalJournal of Histochemistry and Cytochemistry
Volume36
Issue number1
DOIs
StatePublished - Jan 1 1988

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Proteins
Bronchioles
Bronchoalveolar Lavage
Coloring Agents
Uteroglobin
Leukocyte Elastase
Pancreatic Elastase
Concanavalin A
Centrifugation
Aspartic Acid
Leucine
Trypsin
Serine
Chromatography
Immune Sera
Glutamic Acid
Peptide Hydrolases
Dogs
Staining and Labeling
Amino Acids

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Identification, cellular localization, isolation, and characterization of human Clara cell-specific 10 KD protein. / Singh, Gurmukh; Singh, J.; Katyal, S. L.; Brown, W. E.; Kramps, J. A.; Paradis, I. L.; Dauber, J. H.; Macpherson, T. A.; Squeglia, N.

In: Journal of Histochemistry and Cytochemistry, Vol. 36, No. 1, 01.01.1988, p. 73-80.

Research output: Contribution to journalArticle

Singh, G, Singh, J, Katyal, SL, Brown, WE, Kramps, JA, Paradis, IL, Dauber, JH, Macpherson, TA & Squeglia, N 1988, 'Identification, cellular localization, isolation, and characterization of human Clara cell-specific 10 KD protein', Journal of Histochemistry and Cytochemistry, vol. 36, no. 1, pp. 73-80. https://doi.org/10.1177/36.1.3275712
Singh, Gurmukh ; Singh, J. ; Katyal, S. L. ; Brown, W. E. ; Kramps, J. A. ; Paradis, I. L. ; Dauber, J. H. ; Macpherson, T. A. ; Squeglia, N. / Identification, cellular localization, isolation, and characterization of human Clara cell-specific 10 KD protein. In: Journal of Histochemistry and Cytochemistry. 1988 ; Vol. 36, No. 1. pp. 73-80.
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