Identification, isolation, and partial characterization of a 7.5-kda surfactant-associated protein

Gurmukh Singh, Sikandar L. Katyal, William E. Brown, Amy L. Kennedy, Mari Lou Wong-Chong, Shirley A. Gottron

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human surfactant was analyzed for proteins associated with the lipids. Surfactant was isolated from lung lavage by the salt gradient centrifugation method, and the soluble proteins binding to the lipids were recovered by extraction with a low pH buffer. Antiserum to this preparation reacted with surfactant apoprotein A and a 7.5-kDa protein. The 7.5-kDa protein was isolated from reduced and alkylated lung lavage pellet by chromatography. The N-terminal amino acid sequence of the protein indicates that it is a novel protein.

Original languageEnglish (US)
Pages (from-to)559-567
Number of pages9
JournalExperimental Lung Research
Volume17
Issue number3
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

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Surface-Active Agents
Bronchoalveolar Lavage
Proteins
Lipids
Apoproteins
Centrifugation
Chromatography
Protein Binding
Immune Sera
Amino Acid Sequence
Buffers
Salts
Amino Acids

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry

Cite this

Identification, isolation, and partial characterization of a 7.5-kda surfactant-associated protein. / Singh, Gurmukh; Katyal, Sikandar L.; Brown, William E.; Kennedy, Amy L.; Wong-Chong, Mari Lou; Gottron, Shirley A.

In: Experimental Lung Research, Vol. 17, No. 3, 01.01.1991, p. 559-567.

Research output: Contribution to journalArticle

Singh, Gurmukh ; Katyal, Sikandar L. ; Brown, William E. ; Kennedy, Amy L. ; Wong-Chong, Mari Lou ; Gottron, Shirley A. / Identification, isolation, and partial characterization of a 7.5-kda surfactant-associated protein. In: Experimental Lung Research. 1991 ; Vol. 17, No. 3. pp. 559-567.
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