Identification of a cytolytic protein in the coelomic fluid of Eisenia foetida earthworms

Martin Bilej, Lea Brys, Alain Beschin, Rudolf Lucas, Edilbert Vercauteren, Radka Hanušová, Patrick De Baetselier

Research output: Contribution to journalArticle

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Abstract

Total coelomic fluid of earthworms Eisenia foetida (Oligochaeta, Annelida) is capable of lysing different mammalian tumor cell lines. This cytolytic activity is different from tumor necrosis factor (TNF)-mediated lysis and is not due to proteolysis. Total coelomic fluid was subjected to ion-exchange chromatography separation and a fraction with prominent cytolytic activity was used to elicit monoclonal antibodies that were screened for their capacity to neutralize the cytolytic effect of total coelomic fluid. One of the prepared neutralizing IgG antibodies was used for the immunoaffinity purification of a cytolytic factor from total coelomic fluid. SDS-PAGE and Western blot analyses revealed a protein band with an apparent molecular weight of 42 kDa. This cytolytic protein (termed CCF-1 or coelomic cytolytic factor 1) can be adsorbed on the surface of opsonized particles and may be involved in opsonizing and hemolytic effects of coelomic fluid.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalImmunology Letters
Volume45
Issue number1-2
DOIs
StatePublished - Jan 1 1995

Fingerprint

Oligochaeta
Annelida
Proteins
Ion Exchange Chromatography
Neutralizing Antibodies
Tumor Cell Line
Proteolysis
Polyacrylamide Gel Electrophoresis
Tumor Necrosis Factor-alpha
Immunoglobulin G
Molecular Weight
Western Blotting
Monoclonal Antibodies

Keywords

  • Coelomic fluid
  • Cytolytic activity
  • Eisenia foetida
  • Hemolysis
  • Tumor cell line

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Bilej, M., Brys, L., Beschin, A., Lucas, R., Vercauteren, E., Hanušová, R., & De Baetselier, P. (1995). Identification of a cytolytic protein in the coelomic fluid of Eisenia foetida earthworms. Immunology Letters, 45(1-2), 123-128. https://doi.org/10.1016/0165-2478(94)00248-P

Identification of a cytolytic protein in the coelomic fluid of Eisenia foetida earthworms. / Bilej, Martin; Brys, Lea; Beschin, Alain; Lucas, Rudolf; Vercauteren, Edilbert; Hanušová, Radka; De Baetselier, Patrick.

In: Immunology Letters, Vol. 45, No. 1-2, 01.01.1995, p. 123-128.

Research output: Contribution to journalArticle

Bilej, M, Brys, L, Beschin, A, Lucas, R, Vercauteren, E, Hanušová, R & De Baetselier, P 1995, 'Identification of a cytolytic protein in the coelomic fluid of Eisenia foetida earthworms', Immunology Letters, vol. 45, no. 1-2, pp. 123-128. https://doi.org/10.1016/0165-2478(94)00248-P
Bilej, Martin ; Brys, Lea ; Beschin, Alain ; Lucas, Rudolf ; Vercauteren, Edilbert ; Hanušová, Radka ; De Baetselier, Patrick. / Identification of a cytolytic protein in the coelomic fluid of Eisenia foetida earthworms. In: Immunology Letters. 1995 ; Vol. 45, No. 1-2. pp. 123-128.
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