Identification of a differentially expressed RNA helicase by gene trapping

Daniel S. Wagner, Lin Gan, William H. Klein

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A mouse line was generated that expressed a gene trap reporter construct, βgeo, in a dynamic pattern during embryonic development. Differential expression was seen within the developing eyes, limbs, heart, neural tube, and skeleton. Two transcripts were cloned that contained endogenous sequences fused to the gene trap vector sequence. Analysis of the endogenous sequences revealed that the reporter integrated within a gene belonging to a small group of eukaryotic superfamily I helicases. Unexpectedly, the majority of transcripts produced from the trapped locus were not affected by the insertion of the reporter. Although the function of the trapped helicase gene is unknown, its complex transcription patterns and widespread spatial-temporal distribution suggest that the gene product plays a role in RNA metabolism in multiple tissues and organs within the developing embryo.

Original languageEnglish (US)
Pages (from-to)677-684
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume262
Issue number3
DOIs
StatePublished - Sep 7 1999
Externally publishedYes

Keywords

  • Embryonic gene expression
  • Gene trap
  • Helicase
  • Mouse embryogenesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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