Identification of a functional type IA topoisomerase, Ld TopIIIβ, from kinetoplastid parasite Leishmania donovani

Bijoylaxmi Banerjee, Nilkantha Sen, Hemanta K. Majumder

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

DNA topoisomerases of kinetoplastids represent a family of DNA processing enzymes that essentially solve the topological problems not only in nuclear DNA but also in kinetoplast DNA. We have, for the first time, identified a Leishmania donovani homologue of bacterial and eukaryotic IA type of topoisomerase III protein and termed as LdTopIIIβ. Complementation study of wild-type and mutant LdTopIIIβ with slow-growing topoisomerase III mutant yeast S. cerevisiae revealed the functional conservation of the leishmanial counterpart of topoisomerase IIIβ protein, the 327 tyrosine being the active site amino acid. A C-terminal deletion construct of LdTopIIIβ could not suppress the slow-growth phenotype of mutant yeast, indicating the requirement of C-terminal region for the enzyme function in vivo. LdTopIIIβ localized inside the nucleus and kinetoplast of the parasite. Taken together, our study indicates functional conservation and possible role of LdTopIIIβ in parasite DNA processing.

Original languageEnglish (US)
Article number230542
JournalEnzyme Research
Volume2011
Issue number1
DOIs
StatePublished - Dec 1 2011

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Leishmania donovani
Parasites
Yeast
DNA
Conservation
DNA Topoisomerases
Yeasts
Kinetoplast DNA
Enzymes
Processing
Saccharomyces cerevisiae
Tyrosine
Catalytic Domain
Proteins
Phenotype
Amino Acids
Growth
DNA topoisomerase III

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Identification of a functional type IA topoisomerase, Ld TopIIIβ, from kinetoplastid parasite Leishmania donovani. / Banerjee, Bijoylaxmi; Sen, Nilkantha; Majumder, Hemanta K.

In: Enzyme Research, Vol. 2011, No. 1, 230542, 01.12.2011.

Research output: Contribution to journalArticle

Banerjee, Bijoylaxmi ; Sen, Nilkantha ; Majumder, Hemanta K. / Identification of a functional type IA topoisomerase, Ld TopIIIβ, from kinetoplastid parasite Leishmania donovani. In: Enzyme Research. 2011 ; Vol. 2011, No. 1.
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