Identification of a novel alternative splicing isoform of human amyloid precursor protein gene, APP639

Ke Tang, Chen Wang, Chengyong Shen, Shuli Sheng, Rivka Ravid, Naihe Jing

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Senile plaques, neurofibrillary tangles and amyloid-laden cerebral vessels are characteristic features in the brains of individuals with Alzheimer's disease (AD). The principal component of amyloid in senile plaque and amyloid-laden cerebral vessels is β-amyloid (Aβ), a peptide proteolytically derived from a large amyloid precursor protein (APP). To date, several alternatively spliced human APP transcripts have been described. Here, we report the identification of a novel alternative splicing isoform of the human APP gene, APP639, which excludes exon 2 as well as exons 7 and 8. RT-PCR and Southern blot analysis show that APP639 mRNA is expressed in many human fetal tissues. In contrast, the APP639 transcript is hardly detected in the aged human cerebral cortex from both pathologically confirmed sporadic AD cases and nondemented controls. However, APP639 mRNA exists in the adult human liver. Western blot analysis shows that the protein product produced from the APP639 cDNA could be recognized by the APP antibody, and it does lack the exon 2 coding region. These results suggest that APP639, a novel alternative splicing isoform of human APP gene, does exist in human tissues in vivo.

Original languageEnglish (US)
Pages (from-to)102-108
Number of pages7
JournalEuropean Journal of Neuroscience
Volume18
Issue number1
DOIs
StatePublished - Jul 1 2003

Fingerprint

Amyloid beta-Protein Precursor
Alternative Splicing
Protein Isoforms
Amyloid
Genes
Exons
Amyloid Plaques
Alzheimer Disease
Messenger RNA
Neurofibrillary Tangles
Southern Blotting
Cerebral Cortex
Fetus
Complementary DNA
Western Blotting
Polymerase Chain Reaction
Peptides
Antibodies
Liver
Brain

Keywords

  • APP antibody
  • Alternative splicing
  • Alzheimer's disease
  • Human

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Identification of a novel alternative splicing isoform of human amyloid precursor protein gene, APP639. / Tang, Ke; Wang, Chen; Shen, Chengyong; Sheng, Shuli; Ravid, Rivka; Jing, Naihe.

In: European Journal of Neuroscience, Vol. 18, No. 1, 01.07.2003, p. 102-108.

Research output: Contribution to journalArticle

Tang, Ke ; Wang, Chen ; Shen, Chengyong ; Sheng, Shuli ; Ravid, Rivka ; Jing, Naihe. / Identification of a novel alternative splicing isoform of human amyloid precursor protein gene, APP639. In: European Journal of Neuroscience. 2003 ; Vol. 18, No. 1. pp. 102-108.
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