Identification of caveolin and caveolin-related proteins in the brain

Patricia L Cameron, Johnna W. Ruffin, Roni Jacob Bollag, Howard Rasmussen, Richard S Cameron

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Caveolae are 50-100 nm, nonclathrin-coated, flask-shaped plasma membrane microdomains that have been identified in most mammalian cell types, except lymphocytes and neurons. To date, multiple functions have been ascribed to caveolae, including the compartmentalization of lipid and protein components that function in transmembrane signaling events, biosynthetic transport functions, endocytosis, potocytosis, and transcytosis. Caveolin, a 21-24 kDa integral membrane protein, is the principal structural component of caveolae. We have initiated studies to examine the relationship of detergent-insoluble complexes identified in astrocytes to the caveolin-caveolae compartment detected in cells of peripheral tissues. Immunolocalization studies performed in astrocytes reveal caveolin immunoreactivity in regions that correlate well to the distribution of caveolae and caveolin determined in other cell types, and electron microscopic studies reveal multiple clusters of flask-shaped invaginations aligned along the plasma membrane. Immunoblot analyses demonstrate that detergent-insoluble complexes isolated from astrocytes are composed of caveolin-1α, an identification verified by Northern blot analyses and by the cloning of a cDNA using reverse transcriptase-PCR amplification from total astrocyte RNA. Using a full-length caveolin-1 probe, Northern blot analyses suggest that the expression of caveolin-1 may be regulated during brain development. Immunoblot analyses of detergent- insoluble complexes isolated from cerebral cortex and cerebellum identify two immunoreactive polypeptides with apparent molecular weight and isoelectric points appropriate for caveolin. The identification of caveolae microdomains and caveolin-1 in astrocytes and brain, as well as the apparent regulation of caveolin-1 expression during brain development, identifies a cell compartment not detected previously in brain.

Original languageEnglish (US)
Pages (from-to)9520-9535
Number of pages16
JournalJournal of Neuroscience
Volume17
Issue number24
StatePublished - Dec 1 1997

Fingerprint

Caveolins
Caveolae
Caveolin 1
Astrocytes
Brain
Detergents
Northern Blotting
Cell Membrane
Membrane Microdomains
Transcytosis
Isoelectric Point
Endocytosis
Reverse Transcriptase Polymerase Chain Reaction
Cerebral Cortex
Cerebellum
Organism Cloning
Membrane Proteins
Complementary DNA
Molecular Weight
Lymphocytes

Keywords

  • Astroglial cells
  • Caveolae
  • Caveolin
  • Membrane transport
  • Nervous system proteins
  • Plasmalemmal vesicles

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Identification of caveolin and caveolin-related proteins in the brain. / Cameron, Patricia L; Ruffin, Johnna W.; Bollag, Roni Jacob; Rasmussen, Howard; Cameron, Richard S.

In: Journal of Neuroscience, Vol. 17, No. 24, 01.12.1997, p. 9520-9535.

Research output: Contribution to journalArticle

@article{b78d04aaab7f45c395aac8d2bfd6a48f,
title = "Identification of caveolin and caveolin-related proteins in the brain",
abstract = "Caveolae are 50-100 nm, nonclathrin-coated, flask-shaped plasma membrane microdomains that have been identified in most mammalian cell types, except lymphocytes and neurons. To date, multiple functions have been ascribed to caveolae, including the compartmentalization of lipid and protein components that function in transmembrane signaling events, biosynthetic transport functions, endocytosis, potocytosis, and transcytosis. Caveolin, a 21-24 kDa integral membrane protein, is the principal structural component of caveolae. We have initiated studies to examine the relationship of detergent-insoluble complexes identified in astrocytes to the caveolin-caveolae compartment detected in cells of peripheral tissues. Immunolocalization studies performed in astrocytes reveal caveolin immunoreactivity in regions that correlate well to the distribution of caveolae and caveolin determined in other cell types, and electron microscopic studies reveal multiple clusters of flask-shaped invaginations aligned along the plasma membrane. Immunoblot analyses demonstrate that detergent-insoluble complexes isolated from astrocytes are composed of caveolin-1α, an identification verified by Northern blot analyses and by the cloning of a cDNA using reverse transcriptase-PCR amplification from total astrocyte RNA. Using a full-length caveolin-1 probe, Northern blot analyses suggest that the expression of caveolin-1 may be regulated during brain development. Immunoblot analyses of detergent- insoluble complexes isolated from cerebral cortex and cerebellum identify two immunoreactive polypeptides with apparent molecular weight and isoelectric points appropriate for caveolin. The identification of caveolae microdomains and caveolin-1 in astrocytes and brain, as well as the apparent regulation of caveolin-1 expression during brain development, identifies a cell compartment not detected previously in brain.",
keywords = "Astroglial cells, Caveolae, Caveolin, Membrane transport, Nervous system proteins, Plasmalemmal vesicles",
author = "Cameron, {Patricia L} and Ruffin, {Johnna W.} and Bollag, {Roni Jacob} and Howard Rasmussen and Cameron, {Richard S}",
year = "1997",
month = "12",
day = "1",
language = "English (US)",
volume = "17",
pages = "9520--9535",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "24",

}

TY - JOUR

T1 - Identification of caveolin and caveolin-related proteins in the brain

AU - Cameron, Patricia L

AU - Ruffin, Johnna W.

AU - Bollag, Roni Jacob

AU - Rasmussen, Howard

AU - Cameron, Richard S

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Caveolae are 50-100 nm, nonclathrin-coated, flask-shaped plasma membrane microdomains that have been identified in most mammalian cell types, except lymphocytes and neurons. To date, multiple functions have been ascribed to caveolae, including the compartmentalization of lipid and protein components that function in transmembrane signaling events, biosynthetic transport functions, endocytosis, potocytosis, and transcytosis. Caveolin, a 21-24 kDa integral membrane protein, is the principal structural component of caveolae. We have initiated studies to examine the relationship of detergent-insoluble complexes identified in astrocytes to the caveolin-caveolae compartment detected in cells of peripheral tissues. Immunolocalization studies performed in astrocytes reveal caveolin immunoreactivity in regions that correlate well to the distribution of caveolae and caveolin determined in other cell types, and electron microscopic studies reveal multiple clusters of flask-shaped invaginations aligned along the plasma membrane. Immunoblot analyses demonstrate that detergent-insoluble complexes isolated from astrocytes are composed of caveolin-1α, an identification verified by Northern blot analyses and by the cloning of a cDNA using reverse transcriptase-PCR amplification from total astrocyte RNA. Using a full-length caveolin-1 probe, Northern blot analyses suggest that the expression of caveolin-1 may be regulated during brain development. Immunoblot analyses of detergent- insoluble complexes isolated from cerebral cortex and cerebellum identify two immunoreactive polypeptides with apparent molecular weight and isoelectric points appropriate for caveolin. The identification of caveolae microdomains and caveolin-1 in astrocytes and brain, as well as the apparent regulation of caveolin-1 expression during brain development, identifies a cell compartment not detected previously in brain.

AB - Caveolae are 50-100 nm, nonclathrin-coated, flask-shaped plasma membrane microdomains that have been identified in most mammalian cell types, except lymphocytes and neurons. To date, multiple functions have been ascribed to caveolae, including the compartmentalization of lipid and protein components that function in transmembrane signaling events, biosynthetic transport functions, endocytosis, potocytosis, and transcytosis. Caveolin, a 21-24 kDa integral membrane protein, is the principal structural component of caveolae. We have initiated studies to examine the relationship of detergent-insoluble complexes identified in astrocytes to the caveolin-caveolae compartment detected in cells of peripheral tissues. Immunolocalization studies performed in astrocytes reveal caveolin immunoreactivity in regions that correlate well to the distribution of caveolae and caveolin determined in other cell types, and electron microscopic studies reveal multiple clusters of flask-shaped invaginations aligned along the plasma membrane. Immunoblot analyses demonstrate that detergent-insoluble complexes isolated from astrocytes are composed of caveolin-1α, an identification verified by Northern blot analyses and by the cloning of a cDNA using reverse transcriptase-PCR amplification from total astrocyte RNA. Using a full-length caveolin-1 probe, Northern blot analyses suggest that the expression of caveolin-1 may be regulated during brain development. Immunoblot analyses of detergent- insoluble complexes isolated from cerebral cortex and cerebellum identify two immunoreactive polypeptides with apparent molecular weight and isoelectric points appropriate for caveolin. The identification of caveolae microdomains and caveolin-1 in astrocytes and brain, as well as the apparent regulation of caveolin-1 expression during brain development, identifies a cell compartment not detected previously in brain.

KW - Astroglial cells

KW - Caveolae

KW - Caveolin

KW - Membrane transport

KW - Nervous system proteins

KW - Plasmalemmal vesicles

UR - http://www.scopus.com/inward/record.url?scp=0031466385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031466385&partnerID=8YFLogxK

M3 - Article

C2 - 9391007

AN - SCOPUS:0031466385

VL - 17

SP - 9520

EP - 9535

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 24

ER -