Identification of glycoconjugates which are targets for anti-Gal(β1-3)GalNAc autoantibodies in spinal motor neurons

Alessandra Lugaresi, Massimo Corbo, Florian P. Thomas, Noboyuki Miyatani, Toshio Ariga, Robert K Yu, Arthur P. Hays, Norman Latov

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Human IgM anti-Gal(β1-3)GalNAc antibodies which bind to GM1 and GD1b, are implicated in the pathogenesis of predominantly motor neuropathy or motor neuron disease. By immunofluorescence microscopy, the human antibodies immunostain the surface of motor neurons from bovine spinal cord. The motor neurons are also immunostained by cholera toxin (CT), which is specific for GM1. Glycolipid analysis using thin-layer chromatography (TLC) and immunostaining reveals that the relative concentration of GM1 and GD1b in motor neurons is greatly reduced in comparison to whole spinal cord, and that other motor neuron gangliosides are unreactive with the anti-Gal(β1-3)GalNAc antibodies. By Western blot analysis, the antibodies react with several protein bands in motor neuron extracts, and many of the same proteins are also recognized by PNA. These data suggest that both glycoproteins and glycolipids might be targets for anti-Gal(β1-3)GalNAc antibodies in spinal motor neurons.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalJournal of Neuroimmunology
Volume34
Issue number1
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

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Glycoconjugates
Motor Neurons
Autoantibodies
Antibodies
Glycolipids
Spinal Cord
Motor Neuron Disease
Gangliosides
Cholera Toxin
Thin Layer Chromatography
Fluorescence Microscopy
galactosyll(1,3)-N-acetylgalactosamine
Glycoproteins
Proteins
Western Blotting

Keywords

  • Antibody
  • Autoimmunity
  • Gal(β1-3)GalNAc
  • Ganglioside
  • Glycoprotein
  • Motor neuron

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Identification of glycoconjugates which are targets for anti-Gal(β1-3)GalNAc autoantibodies in spinal motor neurons. / Lugaresi, Alessandra; Corbo, Massimo; Thomas, Florian P.; Miyatani, Noboyuki; Ariga, Toshio; Yu, Robert K; Hays, Arthur P.; Latov, Norman.

In: Journal of Neuroimmunology, Vol. 34, No. 1, 01.01.1991, p. 69-76.

Research output: Contribution to journalArticle

Lugaresi, Alessandra ; Corbo, Massimo ; Thomas, Florian P. ; Miyatani, Noboyuki ; Ariga, Toshio ; Yu, Robert K ; Hays, Arthur P. ; Latov, Norman. / Identification of glycoconjugates which are targets for anti-Gal(β1-3)GalNAc autoantibodies in spinal motor neurons. In: Journal of Neuroimmunology. 1991 ; Vol. 34, No. 1. pp. 69-76.
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