Abstract
As part of an ongoing molecular epidemiological investigation of human immunodeficiency virus type 1 (HIV-1) in rural Georgia, the 5′ half of reverse transcriptase (RT) genotypes from 30 patients was sequenced and phylogenetically analyzed. Two patients, GA132 and GA169, were infected with pol sequences of non-B subtype origin that were found to cluster phylogenetically with subtype A-E of Thai origin. Sliding window bootstrap analysis of GA169 showed clear evidence of A/B recombination within the pol gene segment, whereas in the other patient, GA132, no break point within RT could be identified. Interestingly, pairwise comparisons between these 2 patients' C2-V3 env region revealed a 13.5% divergence. However, similar comparisons within the non-B pol segments yielded a 1.23% nucleotide divergence, which suggests a complex phylogenetic and epidemiological history of the subtype A pol genotype in this region. These data demonstrate an increasing diversity of HIV-1 subtypes and the potential emergence of previously unidentified HIV-1 A-E/B recombinants in the rural United States.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 138-142 |
| Number of pages | 5 |
| Journal | Journal of Infectious Diseases |
| Volume | 183 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2001 |
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ASJC Scopus subject areas
- Immunology and Allergy
- Infectious Diseases
Cite this
Identification of non-B human immunodeficiency virus type 1 subtypes in rural Georgia. / Womack, Chad; Roth, William; Newman, Cheryl L; Rissing, John Peter; Lovell, Roger; Haburchak, David R; Essex, Max; Bond, V. Craig.
In: Journal of Infectious Diseases, Vol. 183, No. 1, 01.01.2001, p. 138-142.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of non-B human immunodeficiency virus type 1 subtypes in rural Georgia
AU - Womack, Chad
AU - Roth, William
AU - Newman, Cheryl L
AU - Rissing, John Peter
AU - Lovell, Roger
AU - Haburchak, David R
AU - Essex, Max
AU - Bond, V. Craig
PY - 2001/1/1
Y1 - 2001/1/1
N2 - As part of an ongoing molecular epidemiological investigation of human immunodeficiency virus type 1 (HIV-1) in rural Georgia, the 5′ half of reverse transcriptase (RT) genotypes from 30 patients was sequenced and phylogenetically analyzed. Two patients, GA132 and GA169, were infected with pol sequences of non-B subtype origin that were found to cluster phylogenetically with subtype A-E of Thai origin. Sliding window bootstrap analysis of GA169 showed clear evidence of A/B recombination within the pol gene segment, whereas in the other patient, GA132, no break point within RT could be identified. Interestingly, pairwise comparisons between these 2 patients' C2-V3 env region revealed a 13.5% divergence. However, similar comparisons within the non-B pol segments yielded a 1.23% nucleotide divergence, which suggests a complex phylogenetic and epidemiological history of the subtype A pol genotype in this region. These data demonstrate an increasing diversity of HIV-1 subtypes and the potential emergence of previously unidentified HIV-1 A-E/B recombinants in the rural United States.
AB - As part of an ongoing molecular epidemiological investigation of human immunodeficiency virus type 1 (HIV-1) in rural Georgia, the 5′ half of reverse transcriptase (RT) genotypes from 30 patients was sequenced and phylogenetically analyzed. Two patients, GA132 and GA169, were infected with pol sequences of non-B subtype origin that were found to cluster phylogenetically with subtype A-E of Thai origin. Sliding window bootstrap analysis of GA169 showed clear evidence of A/B recombination within the pol gene segment, whereas in the other patient, GA132, no break point within RT could be identified. Interestingly, pairwise comparisons between these 2 patients' C2-V3 env region revealed a 13.5% divergence. However, similar comparisons within the non-B pol segments yielded a 1.23% nucleotide divergence, which suggests a complex phylogenetic and epidemiological history of the subtype A pol genotype in this region. These data demonstrate an increasing diversity of HIV-1 subtypes and the potential emergence of previously unidentified HIV-1 A-E/B recombinants in the rural United States.
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U2 - 10.1086/317649
DO - 10.1086/317649
M3 - Article
VL - 183
SP - 138
EP - 142
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 1
ER -