Identification of PSA peptide mimotopes using phage display peptide library

Arul Kumaran Shanmugam, Robert Suriano, Devyani Chaudhuri, Shilpi Rajoria, Andrea George, Abraham Mittelman, Raj K. Tiwari

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Prostate cancer (PCa) is one of the most common types of cancer in men in the United States and is the second leading cause of cancer related death in men. Clinically, secreted prostate specific antigen (PSA) has gained recognition because of its proteolytic activity being directly linked to PCa cell proliferation leading to disease initiation and progression. Using phage display technology, we identified four distinct cyclical peptides. These peptides apart from differences in their amino acid sequence, elicited minimal cross reactive antibody responses against each other. One of the four peptides analyzed produced an antibody response that recognizes the PSA protein. We demonstrate that the synthetic PSA peptide mimics identified in our study are immunologically active and produce neutralizing activity and this has relevance and utility for prostate cancer disease progression.

Original languageEnglish (US)
Pages (from-to)1097-1102
Number of pages6
JournalPeptides
Volume32
Issue number6
DOIs
StatePublished - Jun 1 2011

Fingerprint

Peptide Library
Prostate-Specific Antigen
Prostatic Neoplasms
Peptides
Antibody Formation
Disease Progression
Synthetic Vaccines
Bacteriophages
Antibodies
Cell proliferation
Amino Acid Sequence
Neoplasms
Display devices
Cell Proliferation
Technology
Amino Acids
Proteins

Keywords

  • PSA
  • Peptide mimotope
  • Phage display
  • Prostate cancer

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience

Cite this

Shanmugam, A. K., Suriano, R., Chaudhuri, D., Rajoria, S., George, A., Mittelman, A., & Tiwari, R. K. (2011). Identification of PSA peptide mimotopes using phage display peptide library. Peptides, 32(6), 1097-1102. https://doi.org/10.1016/j.peptides.2011.04.018

Identification of PSA peptide mimotopes using phage display peptide library. / Shanmugam, Arul Kumaran; Suriano, Robert; Chaudhuri, Devyani; Rajoria, Shilpi; George, Andrea; Mittelman, Abraham; Tiwari, Raj K.

In: Peptides, Vol. 32, No. 6, 01.06.2011, p. 1097-1102.

Research output: Contribution to journalArticle

Shanmugam, AK, Suriano, R, Chaudhuri, D, Rajoria, S, George, A, Mittelman, A & Tiwari, RK 2011, 'Identification of PSA peptide mimotopes using phage display peptide library', Peptides, vol. 32, no. 6, pp. 1097-1102. https://doi.org/10.1016/j.peptides.2011.04.018
Shanmugam AK, Suriano R, Chaudhuri D, Rajoria S, George A, Mittelman A et al. Identification of PSA peptide mimotopes using phage display peptide library. Peptides. 2011 Jun 1;32(6):1097-1102. https://doi.org/10.1016/j.peptides.2011.04.018
Shanmugam, Arul Kumaran ; Suriano, Robert ; Chaudhuri, Devyani ; Rajoria, Shilpi ; George, Andrea ; Mittelman, Abraham ; Tiwari, Raj K. / Identification of PSA peptide mimotopes using phage display peptide library. In: Peptides. 2011 ; Vol. 32, No. 6. pp. 1097-1102.
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