Identification of quantum dot bioconjugates and cellular protein co-localization by hybrid gel blotting

HongYan Liu, Tania Q. Vu

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

New approaches are needed to address the interaction of nanoparticles and cellular proteins at the molecular level. We present a modification of PAGE co-immunoprecipitation, QD-based PA-AGE electrophoresis blotting, and apply this to identify quantum dot (QD) bioconjugate-cellular protein association. This method provides the capability to isolate and evaluate the action of QD bioconjugate-protein complexes in intact cells and to correlate these identified interactions with their location in cells.

Original languageEnglish (US)
Pages (from-to)1044-1049
Number of pages6
JournalNano Letters
Volume7
Issue number4
DOIs
StatePublished - Apr 1 2007

Fingerprint

Semiconductor quantum dots
Gels
quantum dots
gels
proteins
Proteins
electrophoresis
Electrophoresis
cells
Association reactions
interactions
Nanoparticles
nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Identification of quantum dot bioconjugates and cellular protein co-localization by hybrid gel blotting. / Liu, HongYan; Vu, Tania Q.

In: Nano Letters, Vol. 7, No. 4, 01.04.2007, p. 1044-1049.

Research output: Contribution to journalArticle

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