Identification of stem cells after transplantation

Yingjie Wang, Lan Zhang, Yaohua Pan, Lijuan Chen, Neal Lee Weintraub, Yao Liang Tang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The ability to identify the donor stem cells following transplantation into injured hearts is critical. This is particularly important in evaluating stem cell survival and lineage differentiation into mature cardiovascular cells. Several approaches have been employed for tracking the donor stem cells, including fluorescent dyes and fluorescent protein gene transfer. Here, we will induce a protocol using lentivirus-mediated green fluorescent protein (GFP) to monitor the fate of donor stem cells following transplantation.

Original languageEnglish (US)
Title of host publicationCellular Cardiomyoplasty
Subtitle of host publicationMethods and Protocols
Pages89-94
Number of pages6
DOIs
StatePublished - Jul 18 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1036
ISSN (Print)1064-3745

Fingerprint

Stem Cell Transplantation
Stem Cells
Lentivirus
Cell Lineage
Green Fluorescent Proteins
Fluorescent Dyes
Cell Survival
Proteins

Keywords

  • Green fluorescent protein (GFP)
  • Lentivirus
  • Myocardial infarction (MI)
  • Stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Wang, Y., Zhang, L., Pan, Y., Chen, L., Weintraub, N. L., & Tang, Y. L. (2013). Identification of stem cells after transplantation. In Cellular Cardiomyoplasty: Methods and Protocols (pp. 89-94). (Methods in Molecular Biology; Vol. 1036). https://doi.org/10.1007/978-1-62703-511-8_8

Identification of stem cells after transplantation. / Wang, Yingjie; Zhang, Lan; Pan, Yaohua; Chen, Lijuan; Weintraub, Neal Lee; Tang, Yao Liang.

Cellular Cardiomyoplasty: Methods and Protocols. 2013. p. 89-94 (Methods in Molecular Biology; Vol. 1036).

Research output: Chapter in Book/Report/Conference proceedingChapter

Wang, Y, Zhang, L, Pan, Y, Chen, L, Weintraub, NL & Tang, YL 2013, Identification of stem cells after transplantation. in Cellular Cardiomyoplasty: Methods and Protocols. Methods in Molecular Biology, vol. 1036, pp. 89-94. https://doi.org/10.1007/978-1-62703-511-8_8
Wang Y, Zhang L, Pan Y, Chen L, Weintraub NL, Tang YL. Identification of stem cells after transplantation. In Cellular Cardiomyoplasty: Methods and Protocols. 2013. p. 89-94. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-511-8_8
Wang, Yingjie ; Zhang, Lan ; Pan, Yaohua ; Chen, Lijuan ; Weintraub, Neal Lee ; Tang, Yao Liang. / Identification of stem cells after transplantation. Cellular Cardiomyoplasty: Methods and Protocols. 2013. pp. 89-94 (Methods in Molecular Biology).
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