Identification of stem cells after transplantation

Yingjie Wang, Lan Zhang, Yaohua Pan, Lijuan Chen, Neal Weintraub, Yaoliang Tang

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

The ability to identify the donor stem cells following transplantation into injured hearts is critical. This is particularly important in evaluating stem cell survival and lineage differentiation into mature cardiovascular cells. Several approaches have been employed for tracking the donor stem cells, including fluorescent dyes and fluorescent protein gene transfer. Here, we will induce a protocol using lentivirus-mediated green fluorescent protein (GFP) to monitor the fate of donor stem cells following transplantation.

Original languageEnglish (US)
Title of host publicationCellular Cardiomyoplasty
Subtitle of host publicationMethods and Protocols
Pages89-94
Number of pages6
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1036
ISSN (Print)1064-3745

Keywords

  • Green fluorescent protein (GFP)
  • Lentivirus
  • Myocardial infarction (MI)
  • Stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Wang, Y., Zhang, L., Pan, Y., Chen, L., Weintraub, N., & Tang, Y. (2013). Identification of stem cells after transplantation. In Cellular Cardiomyoplasty: Methods and Protocols (pp. 89-94). (Methods in Molecular Biology; Vol. 1036). https://doi.org/10.1007/978-1-62703-511-8_8