Identification of the cysteine nitrosylation sites in human endothelial nitric oxide synthase

Monorama Tummala, Victor Ryzhov, Kandasamy Ravi, Stephen Matthew Black

Research output: Contribution to journalArticle

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Abstract

S-nitrosylation, or the replacement of the hydrogen atom in the thiol group of cysteine residues by a -NO moiety, is a physiologically important posttranslational modification. In our previous work we have shown that S-nitrosylation is involved in the disruption of the endothelial nitric oxide synthase (eNOS) dimer and that this involves the disruption of the zinc (Zn) tetrathiolate cluster due to the S-nitrosylation of Cysteine 98. However, human eNOS contains 28 other cysteine residues whose potential to undergo S-nitrosylation has not been determined. Thus, the goal of this study was to identify the cysteine residues within eNOS that are susceptible to S-nitrosylation in vitro. To accomplish this, we utilized a modified biotin switch assay. Our modification included the tryptic digestion of the S-nitrosylated eNOS protein to allow the isolation of S-nitrosylated peptides for further identification by mass spectrometry. Our data indicate that multiple cysteine residues are capable of undergoing S-nitrosylation in the presence of an excess of a nitrosylating agent. All these cysteine residues identified were found to be located on the surface of the protein according to the available X-ray structure of the oxygenase domain of eNOS. Among those identified were Cys 93 and 98, the residues involved in the formation of the eNOS dimer through a Zn tetrathiolate cluster. In addition, cysteine residues within the reductase domain were identified as undergoing S-nitrosylation. We identified cysteines 660, 801, and 1113 as capable of undergoing S-nitrosylation. These cysteines are located within regions known to bind flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), and nicotinamide adenine dinucleotide (NADPH) although from our studies their functional significance is unclear. Finally we identified cysteines 852, 975/990, and 1047/1049 as being susceptible to S-nitrosylation. These cysteines are located in regions of eNOS that have not been implicated in any known biochemical functions and the significance of their S-nitrosylation is not clear from this study. Thus, our data indicate that the eNOS protein can be S-nitrosylated at multiple sites other than within the Zn tetrathiolate cluster, suggesting that S-nitrosylation may regulate eNOS function in ways other than simply by inducing dimer collapse.

Original languageEnglish (US)
Pages (from-to)25-33
Number of pages9
JournalDNA and cell biology
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2008

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Nitric Oxide Synthase Type III
Cysteine
Flavin Mononucleotide
Oxygenases
Flavin-Adenine Dinucleotide
Post Translational Protein Processing
Biotin
NADP
Sulfhydryl Compounds
NAD
Digestion
Hydrogen
Mass Spectrometry
Oxidoreductases
Membrane Proteins
Proteins
X-Rays

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Identification of the cysteine nitrosylation sites in human endothelial nitric oxide synthase. / Tummala, Monorama; Ryzhov, Victor; Ravi, Kandasamy; Black, Stephen Matthew.

In: DNA and cell biology, Vol. 27, No. 1, 01.01.2008, p. 25-33.

Research output: Contribution to journalArticle

Tummala, Monorama ; Ryzhov, Victor ; Ravi, Kandasamy ; Black, Stephen Matthew. / Identification of the cysteine nitrosylation sites in human endothelial nitric oxide synthase. In: DNA and cell biology. 2008 ; Vol. 27, No. 1. pp. 25-33.
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