Identification of urocortin mRNA antisense transcripts in rat tissue

Mingxia Shi, Xiaolang Yan, Donna H. Ryan, Ruth Babette Harris

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Urocortin (UCN) has 45% sequence homology with corticotropin releasing factor (CRF) and binds to CRF receptors. We used reverse-transcriptase-polymerase chain reaction to demonstrate the presence of UCN RNA in various brain regions and in peripheral tissues. Ribonuclease protection assay (RPA) using sense and antisense riboprobes demonstrated the presence of a naturally occurring antisense UCN RNA transcript in a number of tissues. Northern blot indicated that the antisense transcript was the same size as sense UCN. RPA, using probes that covered bases 1 to 560 of 579 bp sequence of rat UCN, indicated that the antisense sequence was complementary to sense UCN but did not contain an open reading frame. Sense and antisense UCN RNA were co-expressed in all tissues that contained levels of either transcript detectable by RPA. Sense RNA expression was greater than antisense in the midbrain, the two transcripts were expressed equally in the hypothalamus and antisense was expressed at higher levels than sense in the liver, heart, and skeletal muscle. Antisense RNA expression was stress responsive, suggesting that it may play a role in regulating transcription or translation of UCN mRNA. (C) 2000 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)317-324
Number of pages8
JournalBrain Research Bulletin
Volume53
Issue number3
DOIs
StatePublished - Dec 16 2000
Externally publishedYes

Fingerprint

Urocortins
Messenger RNA
Antisense RNA
Ribonucleases
RNA
Corticotropin-Releasing Hormone Receptors
Corticotropin-Releasing Hormone
Protein Biosynthesis
Sequence Homology
Mesencephalon
Reverse Transcriptase Polymerase Chain Reaction
Northern Blotting
Open Reading Frames
Hypothalamus
Myocardium
Skeletal Muscle

Keywords

  • Muscle
  • Ribonuclease protection assay
  • Stress

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Identification of urocortin mRNA antisense transcripts in rat tissue. / Shi, Mingxia; Yan, Xiaolang; Ryan, Donna H.; Harris, Ruth Babette.

In: Brain Research Bulletin, Vol. 53, No. 3, 16.12.2000, p. 317-324.

Research output: Contribution to journalArticle

Shi, Mingxia ; Yan, Xiaolang ; Ryan, Donna H. ; Harris, Ruth Babette. / Identification of urocortin mRNA antisense transcripts in rat tissue. In: Brain Research Bulletin. 2000 ; Vol. 53, No. 3. pp. 317-324.
@article{21d1a8ccb50648f4bc411e9430af7881,
title = "Identification of urocortin mRNA antisense transcripts in rat tissue",
abstract = "Urocortin (UCN) has 45{\%} sequence homology with corticotropin releasing factor (CRF) and binds to CRF receptors. We used reverse-transcriptase-polymerase chain reaction to demonstrate the presence of UCN RNA in various brain regions and in peripheral tissues. Ribonuclease protection assay (RPA) using sense and antisense riboprobes demonstrated the presence of a naturally occurring antisense UCN RNA transcript in a number of tissues. Northern blot indicated that the antisense transcript was the same size as sense UCN. RPA, using probes that covered bases 1 to 560 of 579 bp sequence of rat UCN, indicated that the antisense sequence was complementary to sense UCN but did not contain an open reading frame. Sense and antisense UCN RNA were co-expressed in all tissues that contained levels of either transcript detectable by RPA. Sense RNA expression was greater than antisense in the midbrain, the two transcripts were expressed equally in the hypothalamus and antisense was expressed at higher levels than sense in the liver, heart, and skeletal muscle. Antisense RNA expression was stress responsive, suggesting that it may play a role in regulating transcription or translation of UCN mRNA. (C) 2000 Elsevier Science Inc.",
keywords = "Muscle, Ribonuclease protection assay, Stress",
author = "Mingxia Shi and Xiaolang Yan and Ryan, {Donna H.} and Harris, {Ruth Babette}",
year = "2000",
month = "12",
day = "16",
doi = "10.1016/S0361-9230(00)00349-X",
language = "English (US)",
volume = "53",
pages = "317--324",
journal = "Brain Research Bulletin",
issn = "0361-9230",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Identification of urocortin mRNA antisense transcripts in rat tissue

AU - Shi, Mingxia

AU - Yan, Xiaolang

AU - Ryan, Donna H.

AU - Harris, Ruth Babette

PY - 2000/12/16

Y1 - 2000/12/16

N2 - Urocortin (UCN) has 45% sequence homology with corticotropin releasing factor (CRF) and binds to CRF receptors. We used reverse-transcriptase-polymerase chain reaction to demonstrate the presence of UCN RNA in various brain regions and in peripheral tissues. Ribonuclease protection assay (RPA) using sense and antisense riboprobes demonstrated the presence of a naturally occurring antisense UCN RNA transcript in a number of tissues. Northern blot indicated that the antisense transcript was the same size as sense UCN. RPA, using probes that covered bases 1 to 560 of 579 bp sequence of rat UCN, indicated that the antisense sequence was complementary to sense UCN but did not contain an open reading frame. Sense and antisense UCN RNA were co-expressed in all tissues that contained levels of either transcript detectable by RPA. Sense RNA expression was greater than antisense in the midbrain, the two transcripts were expressed equally in the hypothalamus and antisense was expressed at higher levels than sense in the liver, heart, and skeletal muscle. Antisense RNA expression was stress responsive, suggesting that it may play a role in regulating transcription or translation of UCN mRNA. (C) 2000 Elsevier Science Inc.

AB - Urocortin (UCN) has 45% sequence homology with corticotropin releasing factor (CRF) and binds to CRF receptors. We used reverse-transcriptase-polymerase chain reaction to demonstrate the presence of UCN RNA in various brain regions and in peripheral tissues. Ribonuclease protection assay (RPA) using sense and antisense riboprobes demonstrated the presence of a naturally occurring antisense UCN RNA transcript in a number of tissues. Northern blot indicated that the antisense transcript was the same size as sense UCN. RPA, using probes that covered bases 1 to 560 of 579 bp sequence of rat UCN, indicated that the antisense sequence was complementary to sense UCN but did not contain an open reading frame. Sense and antisense UCN RNA were co-expressed in all tissues that contained levels of either transcript detectable by RPA. Sense RNA expression was greater than antisense in the midbrain, the two transcripts were expressed equally in the hypothalamus and antisense was expressed at higher levels than sense in the liver, heart, and skeletal muscle. Antisense RNA expression was stress responsive, suggesting that it may play a role in regulating transcription or translation of UCN mRNA. (C) 2000 Elsevier Science Inc.

KW - Muscle

KW - Ribonuclease protection assay

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=0033668957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033668957&partnerID=8YFLogxK

U2 - 10.1016/S0361-9230(00)00349-X

DO - 10.1016/S0361-9230(00)00349-X

M3 - Article

C2 - 11113586

AN - SCOPUS:0033668957

VL - 53

SP - 317

EP - 324

JO - Brain Research Bulletin

JF - Brain Research Bulletin

SN - 0361-9230

IS - 3

ER -