Idiotype usage by polyclonally activated B cells in experimental autoimmunity and infection

M. Sutmuller, J. J. Baelde, Michael P. Madaio, J. A. Bruijn, E. De Heer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Both in animal models and in human systemic lupus erythematosus (SLE) the occurrence of nephritogenic autoantibodies bearing dominant idiotypes has been described. In this study we investigate the relation between the induction pathway of polyclonal B cell activation and the production and glomerular deposition of nephritogenic antibodies with shared dominant idiotype(s). Polyclonal B cell activation was induced in several experimental models characterized by glomerular immune deposit formation. We monitored the occurrence of dominant idiotypes among immunoglobulins deposited in the glomeruli. In addition, we studied the species specificity of the dominant idiotypes, by monitoring their presence in kidney sections of patients with an immunologically mediated kidney disease. Anti-idiotype antisera against two monoclonal anti-DNA autoantibodies were used, derived from MRL-lpr/lpr mice, i.e. clone H241 and clone H130. Autoantibodies with the H241 idiotype were present in immune complex depositions in all experimental models but not in humans. We therefore conclude that the presence of this dominant idiotype is independent of the induction pathway of polyclonal B cell activation. However, autoantibodies bearing the H130 idiotype were only detected in kidney sections of mice with spontaneous lupus.

Original languageEnglish (US)
Pages (from-to)275-280
Number of pages6
JournalClinical and Experimental Immunology
Volume115
Issue number2
DOIs
StatePublished - Feb 3 1999

Fingerprint

Autoimmunity
Autoantibodies
B-Lymphocytes
Infection
Immunoglobulin Idiotypes
Theoretical Models
Clone Cells
Kidney
Species Specificity
Kidney Diseases
Antigen-Antibody Complex
Systemic Lupus Erythematosus
Immune Sera
Animal Models
Antibodies
DNA
anordiol

Keywords

  • Anti-DNA antibodies autoimmunity
  • Idiotype
  • Immune complex glomerulonephritis

ASJC Scopus subject areas

  • Immunology

Cite this

Idiotype usage by polyclonally activated B cells in experimental autoimmunity and infection. / Sutmuller, M.; Baelde, J. J.; Madaio, Michael P.; Bruijn, J. A.; De Heer, E.

In: Clinical and Experimental Immunology, Vol. 115, No. 2, 03.02.1999, p. 275-280.

Research output: Contribution to journalArticle

Sutmuller, M. ; Baelde, J. J. ; Madaio, Michael P. ; Bruijn, J. A. ; De Heer, E. / Idiotype usage by polyclonally activated B cells in experimental autoimmunity and infection. In: Clinical and Experimental Immunology. 1999 ; Vol. 115, No. 2. pp. 275-280.
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