IL-10 supplementation increases Tregs and decreases hypertension in the RUPP rat model of preeclampsia

Ashlyn Harmon, Denise Cornelius, Lorena Amaral, Adrienne Paige, Florian Herse, Tarek Ibrahim, Gerd Wallukat, Jessica Faulkner, Janae Moseley, Ralf Dechend, Babbette LaMarca

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: The reduced uterine perfusion pressure (RUPP) rat model of preeclampsia was used to determine the effects of added interleukin-10 (IL-10) on Tregs and hypertension in response to placental ischemia and how the decrease in these anti-inflammatory factors mediates the pathophysiology of preeclampsia.Methods: IL-10 (2.5 ng/kg/d) was infused via osmotic mini-pump implanted intraperitoneally on day 14 of gestation and, at the same time, the RUPP procedure was performed.Results: IL-10 reduced mean arterial pressure (p < 0.001), decreased CD4+ T cells (p = 0.044), while increasing Tregs (p = 0.043) which led to lower IL-6 and TNF- (p = 0.008 and p = 0.003), reduced AT1-AA production (p < 0.001), and decreased oxidative stress (p = 0.029) in RUPP rats.Conclusion: These data indicate that IL-10 supplementation increases Tregs and helps to balance the altered immune system seen during preeclampsia.

Original languageEnglish (US)
Pages (from-to)291-306
Number of pages16
JournalHypertension in Pregnancy
Volume34
Issue number3
DOIs
StatePublished - Jul 3 2015
Externally publishedYes

Keywords

  • Hypertension
  • inflammation
  • pregnancy

ASJC Scopus subject areas

  • Internal Medicine
  • Obstetrics and Gynecology

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