IL-15 mimics T cell receptor crosslinking in the induction of cellular proliferation, gene expression, and cytotoxicity in CD8+ memory T cells

Kebin Liu, Marta Catalfamo, Yu Li, Pierre A. Henkart, Nan Ping Weng

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Generation of CD8+ memory T cells requires antigenic stimulation through T cell receptor (TCR); however, maintenance of CD8+ memory T cells seems to be mediated by cytokines, such as IL-15, in a TCR-independent manner. Compared with the TCR-induced activation, less is known about the mechanisms of IL-15 action. We report here a comparative and kinetic analysis of the responses of memory phenotype CD8+ T cells to IL-15 or TCR (anti-CD3) stimulation in vitro. These two stimuli induce highly similar responses in memory phenotype CD8+ T cells as measured by cellular proliferation, gene expression changes, synthesis of effector molecules (IFNγ, tumor necrosis factor β, granzyme B, and perforin), and induction of cytotoxicity. From 189 genes/expressed sequence tags (ESTs) whose expression changed in CD8+ memory T cells after IL-15 and anti-CD3 stimulation identified by cDNA microarray analysis, 77% of the genes/ESTs exhibit a highly similar pattern of expression between IL-15 and anti-CD3-treated cells, and only 16% and 7% of the genes/ESTs are differentially expressed in response to IL-15 and anti-CD3 treatments, respectively. These results show that IL-15 and anti-CD3 stimulation induced remarkably similar gene expression and effector function. Thus, IL-15 acts not only as a crucial growth factor but also as an antigen-independent activator of effector functions for CD8+ memory T cells.

Original languageEnglish (US)
Pages (from-to)6192-6197
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number9
DOIs
StatePublished - Apr 30 2002

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Interleukin-15
T-Cell Antigen Receptor
Cell Proliferation
T-Lymphocytes
Gene Expression
Expressed Sequence Tags
Activator Appliances
Genes
Phenotype
Microarray Analysis
Oligonucleotide Array Sequence Analysis
Intercellular Signaling Peptides and Proteins
Tumor Necrosis Factor-alpha
Maintenance
Cytokines
Antigens

ASJC Scopus subject areas

  • General

Cite this

IL-15 mimics T cell receptor crosslinking in the induction of cellular proliferation, gene expression, and cytotoxicity in CD8+ memory T cells. / Liu, Kebin; Catalfamo, Marta; Li, Yu; Henkart, Pierre A.; Weng, Nan Ping.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 9, 30.04.2002, p. 6192-6197.

Research output: Contribution to journalArticle

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