IL-22 regulation of functional gene expression in salivary gland cells

Tegan N. Lavoie, Wendy C. Carcamo, Arun Wanchoo, Ashok Sharma, Afife Gulec, Kathleen M. Berg, Carol M. Stewart, Cuong Q. Nguyen

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

TH17 cells and their associated signature cytokines, IL-17 and IL-22, are highly elevated in primary Sjögren's syndrome (pSjS). The levels of IL-22 present in sera showed significant correlations with many disease parameters, specifically hyposalivation, anti-SSB, anti-SSA/SSB, hypergammaglobulinemia and rheumatoid factor. The present study aims to examine the biological function of IL-22 on human salivary glands. To accomplish the goal, microarray analysis using the HumanHT-12 v4 Expression BeadChip was utilized to determine the biological function of IL-22. Differential expression analyses were conducted using the LIMMA package from the Bioconductor project. MTT assay, flow cytometry and Western blotting were used to identify the function of IL-22 on human salivary gland cells. Results indicate an extensive effect of IL-22 on many major molecular functions including activation of antimicrobial genes and downregulation of immune-associated pathways. Functional studies performed in-vitro using human salivary gland cells treated with IL-22 indicated a direct effect of IL-22 on cell cycling, specifically reducing cellular proliferation at the G2-M phase by activation of STAT3. These results suggest the important role of IL-22 in the salivary gland function. The present study suggests that IL-22 might be involved in regulating inflammation and controlling the cell proliferation in SjS.

Original languageEnglish (US)
Pages (from-to)178-184
Number of pages7
JournalGenomics Data
Volume7
DOIs
StatePublished - Mar 1 2016

Keywords

  • Cytokine
  • Gene expression
  • IL-22
  • Microarray
  • Sjogren's syndrome

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Medicine
  • Genetics

Fingerprint Dive into the research topics of 'IL-22 regulation of functional gene expression in salivary gland cells'. Together they form a unique fingerprint.

  • Cite this

    Lavoie, T. N., Carcamo, W. C., Wanchoo, A., Sharma, A., Gulec, A., Berg, K. M., Stewart, C. M., & Nguyen, C. Q. (2016). IL-22 regulation of functional gene expression in salivary gland cells. Genomics Data, 7, 178-184. https://doi.org/10.1016/j.gdata.2015.11.014