Il-6 and mouse oocyte spindle

Jashoman Banerjee, Rakesh Sharma, Ashok Agarwal, Dhiman Maitra, Michael P. Diamond, Husam M. Abu-Soud

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Interleukin 6 (IL-6) is considered a major indicator of the acute-phase inflammatory response. Endometriosis and pelvic inflammation, diseases that manifest elevated levels of IL-6, are commonly associated with higher infertility. However, the mechanistic link between elevated levels of IL-6 and poor oocyte quality is still unclear. In this work, we explored the direct role of this cytokine as a possible mediator for impaired oocyte spindle and chromosomal structure, which is a critical hurdle in the management of infertility. Metaphase-II mouse oocytes were exposed to recombinant mouse IL-6 (50, 100 and 200 ng/mL) for 30 minutes and subjected to indirect immunofluorescent staining to identify alterations in the microtubule and chromosomal alignment compared to untreated controls. The deterioration in microtubule and chromosomal alignment were evaluated utilizing both fluorescence and confocal microscopy, and were quantitated with a previously reported scoring system. Our results showed that IL-6 caused a dose-dependent deterioration in microtubule and chromosomal alignment in the treated oocytes as compared to the untreated group. Indeed, IL-6 at a concentration as low as 50 ng/mL caused deterioration in the spindle structure in 60% of the oocytes, which increased significantly (P&0.0001) as IL-6 concentration was increased. In conclusion, elevated levels of IL-6 associated with endometriosis and pelvic inflammation may reduce the fertilizing capacity of human oocyte through a mechanism that involves impairment of the microtubule and chromosomal structure.

Original languageEnglish (US)
Article numbere35535
JournalPloS one
Volume7
Issue number4
DOIs
StatePublished - Apr 20 2012

Fingerprint

interleukin-6
Oocytes
Interleukin-6
oocytes
mice
Microtubules
microtubules
Deterioration
Endometriosis
inflammation
deterioration
Infertility
Inflammation
Acute-Phase Reaction
Confocal microscopy
Fluorescence microscopy
Metaphase
Fluorescence Microscopy
Confocal Microscopy
fluorescence microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Banerjee, J., Sharma, R., Agarwal, A., Maitra, D., Diamond, M. P., & Abu-Soud, H. M. (2012). Il-6 and mouse oocyte spindle. PloS one, 7(4), [e35535]. https://doi.org/10.1371/journal.pone.0035535

Il-6 and mouse oocyte spindle. / Banerjee, Jashoman; Sharma, Rakesh; Agarwal, Ashok; Maitra, Dhiman; Diamond, Michael P.; Abu-Soud, Husam M.

In: PloS one, Vol. 7, No. 4, e35535, 20.04.2012.

Research output: Contribution to journalArticle

Banerjee, J, Sharma, R, Agarwal, A, Maitra, D, Diamond, MP & Abu-Soud, HM 2012, 'Il-6 and mouse oocyte spindle', PloS one, vol. 7, no. 4, e35535. https://doi.org/10.1371/journal.pone.0035535
Banerjee J, Sharma R, Agarwal A, Maitra D, Diamond MP, Abu-Soud HM. Il-6 and mouse oocyte spindle. PloS one. 2012 Apr 20;7(4). e35535. https://doi.org/10.1371/journal.pone.0035535
Banerjee, Jashoman ; Sharma, Rakesh ; Agarwal, Ashok ; Maitra, Dhiman ; Diamond, Michael P. ; Abu-Soud, Husam M. / Il-6 and mouse oocyte spindle. In: PloS one. 2012 ; Vol. 7, No. 4.
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