Imaging and Tracking Stem Cell Engraftment in Ischemic Hearts by Near-Infrared Fluorescent Protein (iRFP) Labeling

Xuan Su, Yan Shen, Neal L. Weintraub, Yaoliang Tang

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Stem cell-based therapies hold great promise as alternative therapeutic strategies for various chronic diseases, including ischemic cardiomyopathy. Tracking the engraftment of transplanted stem cells is critical to the assessment of donor cell survival in the host environment. Fluorescent proteins, such as green fluorescent protein (GFP), have been widely used to track the fate of donor cells; however, GFP labeling has limitations with regard to noninvasively measuring cell engraftment in vivo. Our research indicates that near-infrared fluorescent protein (iRFP) labeling offers advantages for noninvasive in vivo imaging and histological assessment. Here, we present a protocol for using the lentiviral vector-mediated iRFP vector system to label and track donor stem cells in ischemic mouse hearts.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages121-129
Number of pages9
DOIs
StatePublished - 2020

Publication series

NameMethods in Molecular Biology
Volume2150
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Green fluorescent protein (GFP)
  • Lentivirus
  • Myocardial infarction
  • Stem cells
  • iRFP

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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