Imbalance of the nerve growth factor and its precursor as a potential biomarker for diabetic retinopathy

B. A. Mysona, S. Matragoon, M. Stephens, I. N. Mohamed, A. Farooq, M. L. Bartasis, A. Y. Fouda, A. Y. Shanab, Diego Gabriel Espinosa Heidmann, A. B. El-Remessy

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Our previous studies have demonstrated that diabetes-induced oxidative stress alters homeostasis of retinal nerve growth factor (NGF) resulting in accumulation of its precursor, proNGF, at the expense of NGF which plays a critical role in preserving neuronal and retinal function. This imbalance coincided with retinal damage in experimental diabetes. Here we test the hypothesis that alteration of proNGF and NGF levels observed in retina and vitreous will be mirrored in serum of diabetic patients. Blood and vitreous samples were collected from patients (diabetic and nondiabetic) undergoing vitrectomy at Georgia Regents University under approved IRB. Levels of proNGF, NGF, and p75 NTR shedding were detected using Western blot analysis. MMP-7 activity was also assayed. Diabetes-induced proNGF expression and impaired NGF expression were observed in vitreous and serum. Vitreous and sera from diabetic patients (n = 11) showed significant 40.8-fold and 3.6-fold increases, respectively, compared to nondiabetics (n = 9). In contrast, vitreous and sera from diabetic patients showed significant 44% and 64% reductions in NGF levels, respectively, compared to nondiabetics. ProNGF to NGF ratios showed significant correlation between vitreous and serum. Further characterization of diabetes-induced imbalance in the proNGF to NGF ratio will facilitate its utility as an early biomarker for diabetic complications.

Original languageEnglish (US)
Article number571456
JournalBioMed Research International
Volume2015
DOIs
StatePublished - Jan 1 2015

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Biomarkers
Nerve Growth Factor
Diabetic Retinopathy
Medical problems
Serum
Oxidative stress
Research Ethics Committees
Vitrectomy
Diabetes Complications
Matrix Metalloproteinases
Retina
Oxidative Stress
Homeostasis
Blood
Western Blotting

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Mysona, B. A., Matragoon, S., Stephens, M., Mohamed, I. N., Farooq, A., Bartasis, M. L., ... El-Remessy, A. B. (2015). Imbalance of the nerve growth factor and its precursor as a potential biomarker for diabetic retinopathy. BioMed Research International, 2015, [571456]. https://doi.org/10.1155/2015/571456

Imbalance of the nerve growth factor and its precursor as a potential biomarker for diabetic retinopathy. / Mysona, B. A.; Matragoon, S.; Stephens, M.; Mohamed, I. N.; Farooq, A.; Bartasis, M. L.; Fouda, A. Y.; Shanab, A. Y.; Espinosa Heidmann, Diego Gabriel; El-Remessy, A. B.

In: BioMed Research International, Vol. 2015, 571456, 01.01.2015.

Research output: Contribution to journalArticle

Mysona, BA, Matragoon, S, Stephens, M, Mohamed, IN, Farooq, A, Bartasis, ML, Fouda, AY, Shanab, AY, Espinosa Heidmann, DG & El-Remessy, AB 2015, 'Imbalance of the nerve growth factor and its precursor as a potential biomarker for diabetic retinopathy', BioMed Research International, vol. 2015, 571456. https://doi.org/10.1155/2015/571456
Mysona, B. A. ; Matragoon, S. ; Stephens, M. ; Mohamed, I. N. ; Farooq, A. ; Bartasis, M. L. ; Fouda, A. Y. ; Shanab, A. Y. ; Espinosa Heidmann, Diego Gabriel ; El-Remessy, A. B. / Imbalance of the nerve growth factor and its precursor as a potential biomarker for diabetic retinopathy. In: BioMed Research International. 2015 ; Vol. 2015.
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