Immune responses of interferon gamma (IFN-γ) knock out mice to repeated Haemaphysalis longicornis (Acari

Ixodidae) nymph infestations

Badgar Battsetseg, Kamilius Mamiro, Noboru Inoue, Levi Makala, Hideyuki Nagasawa, Yoichiro Iwakura, Yutaka Toyoda, Takeshi Mikami, Kozo Fujisaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

To investigate the immunological mechanisms of acquired resistance to tick infestation, interferon gamma (IFN-γ) deficient mice (IFN-γ -/-mice) were used to assess interleukin-4 (IL-4) and antibody production levels against tick salivary gland antigen on three successive infestations with Haemaphysalis longicornis Neumann nymphs. The engorged body weight of the ticks decreased during the second and third infestations. Similar observations were noted in IFN-γ +/+ mice. However, the engorged body weight of the ticks from IFN-γ +/+ mice were considerably lower than those from IFN-γ -/-mice. A marked increase in antibody production during the second and third infestations was observed indicating that IFN-γ -/-mice could acquire immunological resistance against H. longicornis nymphs. Moreover, IL-4 levels were higher during the first and third infestations but decreased during the second infestation. IL-4 levels were significantly higher in IFN-γ -/-mice than in IFN-γ +/+ mice. We have shown here that the statistically significant high IL-4 levels observed in IFN-γ -/-mice may be a result of type 2 helper cell (Th2) polarization. However, the apparently higher IL-4 levels during the first and third infestations and the notable decline during the second infestation suggest that other cytokines or factors in the host immune system may play a part in regulating EL-4 levels.

Original languageEnglish (US)
Pages (from-to)173-176
Number of pages4
JournalJournal of Medical Entomology
Volume39
Issue number1
DOIs
StatePublished - Jan 1 2002

Fingerprint

Haemaphysalis longicornis
Ixodidae
Nymph
interferon-gamma
Knockout Mice
Interferon-gamma
nymphs
Acari
immune response
interleukin-4
Interleukin-4
mice
Ticks
ticks
antibody formation
Antibody Formation
Tick Infestations
Body Weight
tick infestations
body weight

Keywords

  • Acquired immunity
  • Haemaphysalis longicornis
  • IFN-γ -/-mice
  • Th2 polarization

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

Cite this

Immune responses of interferon gamma (IFN-γ) knock out mice to repeated Haemaphysalis longicornis (Acari : Ixodidae) nymph infestations. / Battsetseg, Badgar; Mamiro, Kamilius; Inoue, Noboru; Makala, Levi; Nagasawa, Hideyuki; Iwakura, Yoichiro; Toyoda, Yutaka; Mikami, Takeshi; Fujisaki, Kozo.

In: Journal of Medical Entomology, Vol. 39, No. 1, 01.01.2002, p. 173-176.

Research output: Contribution to journalArticle

Battsetseg, B, Mamiro, K, Inoue, N, Makala, L, Nagasawa, H, Iwakura, Y, Toyoda, Y, Mikami, T & Fujisaki, K 2002, 'Immune responses of interferon gamma (IFN-γ) knock out mice to repeated Haemaphysalis longicornis (Acari: Ixodidae) nymph infestations', Journal of Medical Entomology, vol. 39, no. 1, pp. 173-176. https://doi.org/10.1603/0022-2585-39.1.173
Battsetseg, Badgar ; Mamiro, Kamilius ; Inoue, Noboru ; Makala, Levi ; Nagasawa, Hideyuki ; Iwakura, Yoichiro ; Toyoda, Yutaka ; Mikami, Takeshi ; Fujisaki, Kozo. / Immune responses of interferon gamma (IFN-γ) knock out mice to repeated Haemaphysalis longicornis (Acari : Ixodidae) nymph infestations. In: Journal of Medical Entomology. 2002 ; Vol. 39, No. 1. pp. 173-176.
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