Immune subtraction for improved resolution in serum protein immunofixation electrophoresis and antibody isotype determination in a patient with autoantibody

Research output: Contribution to journalArticlepeer-review

Abstract

Heavy chain isotypes of low level monoclonal immunoglobulins are sometimes obscured in serum immunofixation electrophoresis (SIFE) by a heavy background of polyclonal immunoglobulins. However, accurate determination of the heavy chain isotype is essential for a complete diagnosis, as isotype determination of autoantibodies may have relevance in determining therapeutic procedures. Immune subtraction (IS) was employed in a patient with neuropathy and GD1a autoantibody. IS allowed identification of the cognate heavy chain related to a lambda light chain restriction noted on initial SIFE as well as isotype determination of the autoantibody. Antisera specific to individual heavy and light chains were used for depletion of specific immunoglobulin types. Depletion of kappa light chain associated immunoglobulins allowed unequivocal determination of the isotype of lambda light chain-associated low level monoclonal band to be IgG Lambda. Selective depletion of kappa, lambda, gamma and mu heavy chain immunoglobulins was employed to determine IgG Kappa isotype of the auto-antibody.

Original languageEnglish (US)
Article numbere00240
JournalPractical Laboratory Medicine
Volume26
DOIs
StatePublished - Aug 2021

Keywords

  • Autoantibody
  • Immune subtraction
  • Immunofixation electrophoresis
  • Isotype determination
  • Monoclonal gammopathy
  • Multiple myeloma
  • Neuropathy

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Fingerprint

Dive into the research topics of 'Immune subtraction for improved resolution in serum protein immunofixation electrophoresis and antibody isotype determination in a patient with autoantibody'. Together they form a unique fingerprint.

Cite this