Immunochemical Identification of 2′, 3′‐Cyclic Nucleotide 3′‐Phosphodiesterase in Central and Peripheral Nervous System Myelin, the Wolfgram Protein Fraction, and Bovine Oligodendrocytes

T. J. Sprinkle, H. J. Sheedlo, T. B. Buxton, John Peter Rissing

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38 Citations (Scopus)

Abstract

Abstract: Myelin proteins and the total Wolfgram protein fraction were isolated from the CNS of several mammalian species and characterized with rabbit anti‐bovine 2′, 3′‐cyclic nucleotide 3′‐phosphodiesterase (CNP) antisera after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and electrophoretic transfer to nitrocellulose membranes. The corresponding CNP proteins cross‐reacted across all species examined, suggesting that the CNP amino acid sequence was fairly well conserved in all six species. The same corresponding proteins were also identified immunochemically in the crude total Wolfgram protein fraction in the region of the W1 myelin protein, thus further supporting and extending two different previous reports indicating a relationship between CNP and the W1 protein. In addition to these CNS enzyme sources, peripheral nervous system CNP (rabbit and rat sciatic nerve) was also recognized by these same rabbit anti‐bovine (CNS) CNP antisera. CNP was also detected in freshly isolated delipidated bovine oligodendrocyte membranes. These results suggest that rabbit anti‐bovine CNP antisera may be of use in localization and structural studies of this enzyme in several different species and will permit clear identification of CNP in oligodendrocytes and their isolated membrane fractions.

Original languageEnglish (US)
Pages (from-to)1664-1671
Number of pages8
JournalJournal of Neurochemistry
Volume41
Issue number6
DOIs
StatePublished - Jan 1 1983

Fingerprint

Myelin Proteins
Oligodendroglia
Peripheral Nervous System
Neurology
Immune Sera
Nucleotides
Central Nervous System
Rabbits
Membranes
Proteins
Collodion
Enzymes
Electrophoresis
Rats
Sciatic Nerve
Gels
Sodium
Amino Acids
Amino Acid Sequence
Wolfgram proteins

Keywords

  • CNP
  • CNS
  • Immunoblot
  • Myelin
  • Oligodendrocytes
  • PNS

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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title = "Immunochemical Identification of 2′, 3′‐Cyclic Nucleotide 3′‐Phosphodiesterase in Central and Peripheral Nervous System Myelin, the Wolfgram Protein Fraction, and Bovine Oligodendrocytes",
abstract = "Abstract: Myelin proteins and the total Wolfgram protein fraction were isolated from the CNS of several mammalian species and characterized with rabbit anti‐bovine 2′, 3′‐cyclic nucleotide 3′‐phosphodiesterase (CNP) antisera after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and electrophoretic transfer to nitrocellulose membranes. The corresponding CNP proteins cross‐reacted across all species examined, suggesting that the CNP amino acid sequence was fairly well conserved in all six species. The same corresponding proteins were also identified immunochemically in the crude total Wolfgram protein fraction in the region of the W1 myelin protein, thus further supporting and extending two different previous reports indicating a relationship between CNP and the W1 protein. In addition to these CNS enzyme sources, peripheral nervous system CNP (rabbit and rat sciatic nerve) was also recognized by these same rabbit anti‐bovine (CNS) CNP antisera. CNP was also detected in freshly isolated delipidated bovine oligodendrocyte membranes. These results suggest that rabbit anti‐bovine CNP antisera may be of use in localization and structural studies of this enzyme in several different species and will permit clear identification of CNP in oligodendrocytes and their isolated membrane fractions.",
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AU - Sheedlo, H. J.

AU - Buxton, T. B.

AU - Rissing, John Peter

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AB - Abstract: Myelin proteins and the total Wolfgram protein fraction were isolated from the CNS of several mammalian species and characterized with rabbit anti‐bovine 2′, 3′‐cyclic nucleotide 3′‐phosphodiesterase (CNP) antisera after sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and electrophoretic transfer to nitrocellulose membranes. The corresponding CNP proteins cross‐reacted across all species examined, suggesting that the CNP amino acid sequence was fairly well conserved in all six species. The same corresponding proteins were also identified immunochemically in the crude total Wolfgram protein fraction in the region of the W1 myelin protein, thus further supporting and extending two different previous reports indicating a relationship between CNP and the W1 protein. In addition to these CNS enzyme sources, peripheral nervous system CNP (rabbit and rat sciatic nerve) was also recognized by these same rabbit anti‐bovine (CNS) CNP antisera. CNP was also detected in freshly isolated delipidated bovine oligodendrocyte membranes. These results suggest that rabbit anti‐bovine CNP antisera may be of use in localization and structural studies of this enzyme in several different species and will permit clear identification of CNP in oligodendrocytes and their isolated membrane fractions.

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