Immunocytochemical evidence of Tulp1-dependent outer segment protein transport pathways in photoreceptor cells

Gregory H. Grossman, Rao F. Watson, Gayle J.T. Pauer, Kathryn Elizabeth Bollinger, Stephanie A. Hagstrom

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Tulp1 is a protein of unknown function exclusive to rod and cone photoreceptor cells. Mutations in the gene cause autosomal recessive retinitis pigmentosa in humans and photoreceptor degeneration in mice. In tulp1-/- mice, rod and cone opsins are mislocalized, and rhodopsin-bearing extracellular vesicles accumulate around the inner segment, indicating that Tulp1 is involved in protein transport from the inner segment to the outer segment. To investigate this further, we sought to define which outer segment transport pathways are Tulp1-dependent. We used immunohistochemistry to examine the localization of outer segment proteins in tulp1-/- photoreceptors, prior to retinal degeneration. We also surveyed the condition of inner segment organelles and rhodopsin transport machinery proteins. Herein, we show that guanylate cyclase 1 and guanylate cyclase activating proteins 1 and 2 are mislocalized in the absence of Tulp1. Furthermore, arrestin does not translocate to the outer segment in response to light stimulation. Additionally, data from the tulp1-/- retina adds to the understanding of peripheral membrane protein transport, indicating that rhodopsin kinase and transducin do not co-transport in rhodopsin carrier vesicles and phosphodiesterase does not co-transport in guanylate cyclase carrier vesicles. These data implicate Tulp1 in the transport of selective integral membrane outer segment proteins and their associated proteins, specifically, the opsin and guanylate cyclase carrier pathways. The exact role of Tulp1 in outer segment protein transport remains elusive. However, without Tulp1, two rhodopsin transport machinery proteins exhibit abnormal distribution, Rab8 and Rab11, suggesting a role for Tulp1 in vesicular docking and fusion at the plasma membrane near the connecting cilium.

Original languageEnglish (US)
Pages (from-to)658-668
Number of pages11
JournalExperimental Eye Research
Volume93
Issue number5
DOIs
StatePublished - Nov 1 2011
Externally publishedYes

Fingerprint

Photoreceptor Cells
Rhodopsin
Protein Transport
Guanylate Cyclase-Activating Proteins
Vertebrate Photoreceptor Cells
Guanylate Cyclase
Rod Opsins
Carrier Proteins
Proteins
G-Protein-Coupled Receptor Kinase 1
Cone Opsins
Transducin
Retinal Cone Photoreceptor Cells
Opsins
Arrestin
Retinal Rod Photoreceptor Cells
Retinal Degeneration
Retinitis Pigmentosa
Membrane Transport Proteins
Cilia

Keywords

  • Mouse mutant
  • Photoreceptor
  • Protein transport
  • Retinal degeneration
  • Retinitis pigmentosa
  • Rhodopsin
  • Tulp1

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Immunocytochemical evidence of Tulp1-dependent outer segment protein transport pathways in photoreceptor cells. / Grossman, Gregory H.; Watson, Rao F.; Pauer, Gayle J.T.; Bollinger, Kathryn Elizabeth; Hagstrom, Stephanie A.

In: Experimental Eye Research, Vol. 93, No. 5, 01.11.2011, p. 658-668.

Research output: Contribution to journalArticle

Grossman, Gregory H. ; Watson, Rao F. ; Pauer, Gayle J.T. ; Bollinger, Kathryn Elizabeth ; Hagstrom, Stephanie A. / Immunocytochemical evidence of Tulp1-dependent outer segment protein transport pathways in photoreceptor cells. In: Experimental Eye Research. 2011 ; Vol. 93, No. 5. pp. 658-668.
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