Immunocytochemical study of insulin - like growth factor I and insulin - like growth factor I receptor in human endometrium during the normal menstrual cycle

Marc G. Villeneuve, Leo Plouffe, Cooley G. Pantazis, Michael T. Smith, William O. Thompson, Paul G. McDonough

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Our aims were to detect, using immunocytochemistry, IGFI and IGFI R in the human endometrium and to assess semiquantitatively their levels in the phases of the normal menstrual cycle. Twelve normal proliferative and 10 normal secretory endometrial samples were studied. Each specimen was subjected to an immunocytochemical peroxidase antiperoxidase protocol. The antibodies used to detect IGFI and IGFI R were, respectively. 3D1/2/1 and alpha 1R3. Analysis of variance (ANOVA) was performed to evaluate mean IGFI and IGFI R levels in the glandular epithelium and stroma of each sample while correcting for intra- and interohserver variation. These experiments show the presence of IGFI and IGFI R in human endometrium. There are significant variations in the IGFI and IGFI R levels from patient to patient within each cycle phase, and between glands and stroma within each sample. These findings highlight the importance of the use of in situ studies to clarify endometrial IGFI and IGFI R physiology.

Original languageEnglish (US)
Pages (from-to)180-185
Number of pages6
JournalInternational Journal of Gynecological Pathology
Volume12
Issue number2
DOIs
StatePublished - Jan 1 1993

Fingerprint

IGF Type 1 Receptor
Menstrual Cycle
Endometrium
Insulin-Like Growth Factor I
Peroxidase
Analysis of Variance
Epithelium
Immunohistochemistry
Antibodies

Keywords

  • Human endometrium
  • Immunocytochemistry
  • Insulin-like growth factor I
  • Insulin-like growth factor I receptor

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Obstetrics and Gynecology

Cite this

Immunocytochemical study of insulin - like growth factor I and insulin - like growth factor I receptor in human endometrium during the normal menstrual cycle. / Villeneuve, Marc G.; Plouffe, Leo; Pantazis, Cooley G.; Smith, Michael T.; Thompson, William O.; McDonough, Paul G.

In: International Journal of Gynecological Pathology, Vol. 12, No. 2, 01.01.1993, p. 180-185.

Research output: Contribution to journalArticle

Villeneuve, Marc G. ; Plouffe, Leo ; Pantazis, Cooley G. ; Smith, Michael T. ; Thompson, William O. ; McDonough, Paul G. / Immunocytochemical study of insulin - like growth factor I and insulin - like growth factor I receptor in human endometrium during the normal menstrual cycle. In: International Journal of Gynecological Pathology. 1993 ; Vol. 12, No. 2. pp. 180-185.
@article{c47988a8aa6b4551b6892a437e3cc926,
title = "Immunocytochemical study of insulin - like growth factor I and insulin - like growth factor I receptor in human endometrium during the normal menstrual cycle",
abstract = "Our aims were to detect, using immunocytochemistry, IGFI and IGFI R in the human endometrium and to assess semiquantitatively their levels in the phases of the normal menstrual cycle. Twelve normal proliferative and 10 normal secretory endometrial samples were studied. Each specimen was subjected to an immunocytochemical peroxidase antiperoxidase protocol. The antibodies used to detect IGFI and IGFI R were, respectively. 3D1/2/1 and alpha 1R3. Analysis of variance (ANOVA) was performed to evaluate mean IGFI and IGFI R levels in the glandular epithelium and stroma of each sample while correcting for intra- and interohserver variation. These experiments show the presence of IGFI and IGFI R in human endometrium. There are significant variations in the IGFI and IGFI R levels from patient to patient within each cycle phase, and between glands and stroma within each sample. These findings highlight the importance of the use of in situ studies to clarify endometrial IGFI and IGFI R physiology.",
keywords = "Human endometrium, Immunocytochemistry, Insulin-like growth factor I, Insulin-like growth factor I receptor",
author = "Villeneuve, {Marc G.} and Leo Plouffe and Pantazis, {Cooley G.} and Smith, {Michael T.} and Thompson, {William O.} and McDonough, {Paul G.}",
year = "1993",
month = "1",
day = "1",
doi = "10.1097/00004347-199304000-00017",
language = "English (US)",
volume = "12",
pages = "180--185",
journal = "International Journal of Gynecological Pathology",
issn = "0277-1691",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Immunocytochemical study of insulin - like growth factor I and insulin - like growth factor I receptor in human endometrium during the normal menstrual cycle

AU - Villeneuve, Marc G.

AU - Plouffe, Leo

AU - Pantazis, Cooley G.

AU - Smith, Michael T.

AU - Thompson, William O.

AU - McDonough, Paul G.

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Our aims were to detect, using immunocytochemistry, IGFI and IGFI R in the human endometrium and to assess semiquantitatively their levels in the phases of the normal menstrual cycle. Twelve normal proliferative and 10 normal secretory endometrial samples were studied. Each specimen was subjected to an immunocytochemical peroxidase antiperoxidase protocol. The antibodies used to detect IGFI and IGFI R were, respectively. 3D1/2/1 and alpha 1R3. Analysis of variance (ANOVA) was performed to evaluate mean IGFI and IGFI R levels in the glandular epithelium and stroma of each sample while correcting for intra- and interohserver variation. These experiments show the presence of IGFI and IGFI R in human endometrium. There are significant variations in the IGFI and IGFI R levels from patient to patient within each cycle phase, and between glands and stroma within each sample. These findings highlight the importance of the use of in situ studies to clarify endometrial IGFI and IGFI R physiology.

AB - Our aims were to detect, using immunocytochemistry, IGFI and IGFI R in the human endometrium and to assess semiquantitatively their levels in the phases of the normal menstrual cycle. Twelve normal proliferative and 10 normal secretory endometrial samples were studied. Each specimen was subjected to an immunocytochemical peroxidase antiperoxidase protocol. The antibodies used to detect IGFI and IGFI R were, respectively. 3D1/2/1 and alpha 1R3. Analysis of variance (ANOVA) was performed to evaluate mean IGFI and IGFI R levels in the glandular epithelium and stroma of each sample while correcting for intra- and interohserver variation. These experiments show the presence of IGFI and IGFI R in human endometrium. There are significant variations in the IGFI and IGFI R levels from patient to patient within each cycle phase, and between glands and stroma within each sample. These findings highlight the importance of the use of in situ studies to clarify endometrial IGFI and IGFI R physiology.

KW - Human endometrium

KW - Immunocytochemistry

KW - Insulin-like growth factor I

KW - Insulin-like growth factor I receptor

UR - http://www.scopus.com/inward/record.url?scp=0027499116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027499116&partnerID=8YFLogxK

U2 - 10.1097/00004347-199304000-00017

DO - 10.1097/00004347-199304000-00017

M3 - Article

C2 - 8463043

AN - SCOPUS:0027499116

VL - 12

SP - 180

EP - 185

JO - International Journal of Gynecological Pathology

JF - International Journal of Gynecological Pathology

SN - 0277-1691

IS - 2

ER -