Immunodiagnosis of mesothelioma. Use of antimesothelial cell serum in an indirect immunofluorescence assay

Gurmukh Singh, Theresa L. Whiteside, Andrew Dekker

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Cells isolated from human serous effusions were cultured in vitro. Monolayers of large multipolar cells were established. Antisera to the cultured cells were prepared in rabbits and rats. The antisera were absorbed with human red cells, liver powder and MOLT‐4F cell line lymphocytes. Specificity of the absorbed antisera for human mesothelial cells was demonstrated in an indirect immunofluorescence assay. The antisera were used to confirm the diagnosis of mesothelioma in two cases. In both the patients, the morphologically identifiable malignant cell populations in the effusions stained positively with the anti‐mesothelial cell serum thus establishing their mesothelial origin. Normal nonmesothelial tissue and known nonmesothelial tumors failed to react with the antisera thus confirming the specificity of the antisera.

Original languageEnglish (US)
Pages (from-to)2288-2296
Number of pages9
JournalCancer
Volume43
Issue number6
DOIs
StatePublished - Jan 1 1979

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Immunologic Tests
Mesothelioma
Indirect Fluorescent Antibody Technique
Immune Sera
Serum
Powders
Cultured Cells
Lymphocytes
Rabbits
Cell Line
Liver
Population
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Immunodiagnosis of mesothelioma. Use of antimesothelial cell serum in an indirect immunofluorescence assay. / Singh, Gurmukh; Whiteside, Theresa L.; Dekker, Andrew.

In: Cancer, Vol. 43, No. 6, 01.01.1979, p. 2288-2296.

Research output: Contribution to journalArticle

Singh, Gurmukh ; Whiteside, Theresa L. ; Dekker, Andrew. / Immunodiagnosis of mesothelioma. Use of antimesothelial cell serum in an indirect immunofluorescence assay. In: Cancer. 1979 ; Vol. 43, No. 6. pp. 2288-2296.
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