Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Müller and ganglion cells

R. Krishnan Kutty, William Samuel, Shanyi Chen, Camasamudram Vijayasarathy, Ying Dun, Barbara A Mysona, Barbara Wiggert, Sylvia B Smith

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Novel retinal pigment epithelial cell gene (Norpeg, Rai14), a developmentally regulated mouse gene, encodes a protein containing six ankyrin repeats and a coiled-coil domain. The objective of the present study was to characterize the Norpeg protein and to analyze its expression in mouse retina using an antibody preparation that we developed. The ∼110 kDa Norpeg was immunoprecipitated and characterized by mass spectrometry. Primary cultures of Müller and ganglion cells isolated from the mouse retina were found to express Norpeg when analyzed by immunoblotting and immunofluorescence microscopy. Immunofluorescence analysis of normal mouse retina sections demonstrated that Norpeg is expressed in cells of the ganglion cell layer, inner nuclear layer as well as in the retinal pigment epithelium. Immunoreactivity was also evident in the radial glial (Müller) cell fibers.

Original languageEnglish (US)
Pages (from-to)294-298
Number of pages5
JournalNeuroscience Letters
Volume404
Issue number3
DOIs
StatePublished - Sep 1 2006

Fingerprint

Retinal Ganglion Cells
Fluorescent Antibody Technique
Retina
Ganglia
Ankyrin Repeat
Ependymoglial Cells
Retinal Pigments
Retinal Pigment Epithelium
Fluorescence Microscopy
Immunoblotting
Genes
Mass Spectrometry
Proteins
Epithelial Cells
Antibodies

Keywords

  • Ganglion cells
  • Immunofluorescence
  • Müller cells
  • Norpeg
  • Rai14
  • Retina

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Müller and ganglion cells. / Kutty, R. Krishnan; Samuel, William; Chen, Shanyi; Vijayasarathy, Camasamudram; Dun, Ying; Mysona, Barbara A; Wiggert, Barbara; Smith, Sylvia B.

In: Neuroscience Letters, Vol. 404, No. 3, 01.09.2006, p. 294-298.

Research output: Contribution to journalArticle

Kutty, R. Krishnan ; Samuel, William ; Chen, Shanyi ; Vijayasarathy, Camasamudram ; Dun, Ying ; Mysona, Barbara A ; Wiggert, Barbara ; Smith, Sylvia B. / Immunofluorescence analysis of the expression of Norpeg (Rai14) in retinal Müller and ganglion cells. In: Neuroscience Letters. 2006 ; Vol. 404, No. 3. pp. 294-298.
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