Immunohistochemical analysis of collagen fibrils within the hybrid layer: A FEISEM study

L. Breschi, C. Prati, P. Gobbi, D. Pashley, G. Mazzotti, G. Teti, J. Perdigão

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

This study evaluated the immunohistochemical labeling pattern of dentin collagen fibrils within hybrid layers created by different bonding systems using high resolution SEM. Four different adhesive materials, including self-etching and total-etching systems, were examined: Prime & Bond NT, OptiBond SOLO Plus, Single Bond and Clearfil Protect Bond. All materials were applied to the dentin of extracted human third molars. After cutting the bonded specimens transversely, an anti-collagen type I antibody was incubated on the surface of the dentin-adhesive interface and gold-conjugated secondary antibody was applied to reveal collagen labeling under high resolution SEM. The hybrid layers showed a significant number of collagen fibrils embedded in the resin matrix. The presence of exposed dentin collagen fibrils, as determined by positive labeling with an anti-type I collagen monoclonal antibody, is considered an indication of the presence of incompletely embedded fibrils and, thus, the quality of dentin matrix hybridization. The hybrid layers produced by total-etching systems showed higher labeling compared to those produced by the self-etching system. Positive collagen labeling was also found along the resin tags produced by total-etching adhesive systems.

Original languageEnglish (US)
Pages (from-to)538-546
Number of pages9
JournalOperative dentistry
Volume29
Issue number5
StatePublished - Sep 1 2004

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Dentin
Collagen
Adhesives
Collagen Type I
Third Molar
Antibodies
Gold
Monoclonal Antibodies

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Breschi, L., Prati, C., Gobbi, P., Pashley, D., Mazzotti, G., Teti, G., & Perdigão, J. (2004). Immunohistochemical analysis of collagen fibrils within the hybrid layer: A FEISEM study. Operative dentistry, 29(5), 538-546.

Immunohistochemical analysis of collagen fibrils within the hybrid layer : A FEISEM study. / Breschi, L.; Prati, C.; Gobbi, P.; Pashley, D.; Mazzotti, G.; Teti, G.; Perdigão, J.

In: Operative dentistry, Vol. 29, No. 5, 01.09.2004, p. 538-546.

Research output: Contribution to journalArticle

Breschi, L, Prati, C, Gobbi, P, Pashley, D, Mazzotti, G, Teti, G & Perdigão, J 2004, 'Immunohistochemical analysis of collagen fibrils within the hybrid layer: A FEISEM study', Operative dentistry, vol. 29, no. 5, pp. 538-546.
Breschi L, Prati C, Gobbi P, Pashley D, Mazzotti G, Teti G et al. Immunohistochemical analysis of collagen fibrils within the hybrid layer: A FEISEM study. Operative dentistry. 2004 Sep 1;29(5):538-546.
Breschi, L. ; Prati, C. ; Gobbi, P. ; Pashley, D. ; Mazzotti, G. ; Teti, G. ; Perdigão, J. / Immunohistochemical analysis of collagen fibrils within the hybrid layer : A FEISEM study. In: Operative dentistry. 2004 ; Vol. 29, No. 5. pp. 538-546.
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