Immunohistochemistry of a human type I pneumocyte‐associated protein in lung

Jagjit Singh, Gurmukh Singh, Sikandar L. Katyal, Mari‐Lou ‐L Wong‐Chong, Carol A. McCloskey, Shirley A. Gottron

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A mouse monoclonal antibody to a human lung lavage protein was raised using proteins, with the potential ability to bind surfactant, as the immunogen. The proteins were isolated from cadaver lung lavage. The antibody was tested for its reactivity with lung and other organs. It reacted with type I pneumocytes and some of the nonciliated cells in the surface epithelium of distal bronchioles. Staining was also seen in the cells surrounding the glandular structures, superficial keratinocytes of the skin, endothelium, and nerve sheath cells. With the exception of bronchiolar cells, the stained cells have a squamous morphology, and this protein may serve as a marker or determinant of this characteristic of cells. In pathologic lungs some of the cells in air spaces with “bronchiolarization” of the epithelium exhibited staining for the protein. It could not be ascertained whether the stained cuboidal cells were reactive type II pneumocytes or distal bronchiolar cells. The intraalveolar material in pulmonary alveolar proteinosis did not show remarkable staining for the protein. Even though the protein is not unique to type I pneumocytes, it may serve as a marker for these cells in the study of their development and biology. © 1993 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)357-365
Number of pages9
JournalMicroscopy Research and Technique
Volume26
Issue number5
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

lungs
Immunohistochemistry
proteins
Proteins
Lung
cells
Alveolar Epithelial Cells
staining
Bronchoalveolar Lavage
epithelium
Staining and Labeling
antibodies
markers
Epithelium
Monoclonal antibodies
Pulmonary Alveolar Proteinosis
Bronchioles
Antibodies
endothelium
airspace

Keywords

  • Plasma membrane
  • Squamous cells
  • Surfactant

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Instrumentation
  • Medical Laboratory Technology

Cite this

Singh, J., Singh, G., Katyal, S. L., Wong‐Chong, ML. L., McCloskey, C. A., & Gottron, S. A. (1993). Immunohistochemistry of a human type I pneumocyte‐associated protein in lung. Microscopy Research and Technique, 26(5), 357-365. https://doi.org/10.1002/jemt.1070260503

Immunohistochemistry of a human type I pneumocyte‐associated protein in lung. / Singh, Jagjit; Singh, Gurmukh; Katyal, Sikandar L.; Wong‐Chong, Mari‐Lou ‐L; McCloskey, Carol A.; Gottron, Shirley A.

In: Microscopy Research and Technique, Vol. 26, No. 5, 01.01.1993, p. 357-365.

Research output: Contribution to journalArticle

Singh, J, Singh, G, Katyal, SL, Wong‐Chong, MLL, McCloskey, CA & Gottron, SA 1993, 'Immunohistochemistry of a human type I pneumocyte‐associated protein in lung', Microscopy Research and Technique, vol. 26, no. 5, pp. 357-365. https://doi.org/10.1002/jemt.1070260503
Singh, Jagjit ; Singh, Gurmukh ; Katyal, Sikandar L. ; Wong‐Chong, Mari‐Lou ‐L ; McCloskey, Carol A. ; Gottron, Shirley A. / Immunohistochemistry of a human type I pneumocyte‐associated protein in lung. In: Microscopy Research and Technique. 1993 ; Vol. 26, No. 5. pp. 357-365.
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