Immunostimulant adjuvant patch enhances humoral and cellular immune responses to DNA immunization

Mikayel Mkrtichyan, Anahit Ghochikyan, Nina Movsesyan, Adrine Karapetyan, Gayane Begoyan, Jianmei Yu, Gregory M. Glenn, Ted M. Ross, Michael G. Agadjanyan, David H. Cribbs

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The focus of this report is on the development of an improved DNA immunization protocol, which takes advantage of the strengths of DNA immunization, as well as those associated with adjuvant delivered by transcutaneous immunostimulatory (IS) patches. Because transcutaneous delivery of adjuvants to the skin at the vaccination site has been shown to amplify the immune response to protein antigens, we hypothesized that the same IS patch when placed on the skin at the site of DNA injection could further enhance the immune response to a DNA influenza vaccine. We have combined an influenza DNA vaccine, hemagglutinin fused with three copies of complement C3d, to enhance uptake and antigen presentation, with an IS patch containing heat-labile enterotoxin from Escherichia coli. Coadministration of a potent adjuvant in IS patches placed on the skin at the site of DNA vaccination dramatically amplifies anti-influenza antibody immune response. Supplementing DNA vaccines with IS patches may be a particularly valuable strategy because DNA vaccines can be rapidly modified in response to mutations in pathogens, and individuals with compromised immune systems such as transplant patients and the elderly will benefit from the enhanced antibody response induced by the IS patches.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalDNA and cell biology
Volume27
Issue number1
DOIs
StatePublished - Jan 1 2008

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Immunologic Adjuvants
DNA Vaccines
Humoral Immunity
Cellular Immunity
Immunization
Influenza Vaccines
DNA
Skin
Antibody Formation
Complement C3d
Vaccination
Enterotoxins
Antigen Presentation
Hemagglutinins
Human Influenza
Anti-Idiotypic Antibodies
Immune System
Hot Temperature
Escherichia coli
Transplants

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Mkrtichyan, M., Ghochikyan, A., Movsesyan, N., Karapetyan, A., Begoyan, G., Yu, J., ... Cribbs, D. H. (2008). Immunostimulant adjuvant patch enhances humoral and cellular immune responses to DNA immunization. DNA and cell biology, 27(1), 19-24. https://doi.org/10.1089/dna.2007.0639

Immunostimulant adjuvant patch enhances humoral and cellular immune responses to DNA immunization. / Mkrtichyan, Mikayel; Ghochikyan, Anahit; Movsesyan, Nina; Karapetyan, Adrine; Begoyan, Gayane; Yu, Jianmei; Glenn, Gregory M.; Ross, Ted M.; Agadjanyan, Michael G.; Cribbs, David H.

In: DNA and cell biology, Vol. 27, No. 1, 01.01.2008, p. 19-24.

Research output: Contribution to journalArticle

Mkrtichyan, M, Ghochikyan, A, Movsesyan, N, Karapetyan, A, Begoyan, G, Yu, J, Glenn, GM, Ross, TM, Agadjanyan, MG & Cribbs, DH 2008, 'Immunostimulant adjuvant patch enhances humoral and cellular immune responses to DNA immunization', DNA and cell biology, vol. 27, no. 1, pp. 19-24. https://doi.org/10.1089/dna.2007.0639
Mkrtichyan M, Ghochikyan A, Movsesyan N, Karapetyan A, Begoyan G, Yu J et al. Immunostimulant adjuvant patch enhances humoral and cellular immune responses to DNA immunization. DNA and cell biology. 2008 Jan 1;27(1):19-24. https://doi.org/10.1089/dna.2007.0639
Mkrtichyan, Mikayel ; Ghochikyan, Anahit ; Movsesyan, Nina ; Karapetyan, Adrine ; Begoyan, Gayane ; Yu, Jianmei ; Glenn, Gregory M. ; Ross, Ted M. ; Agadjanyan, Michael G. ; Cribbs, David H. / Immunostimulant adjuvant patch enhances humoral and cellular immune responses to DNA immunization. In: DNA and cell biology. 2008 ; Vol. 27, No. 1. pp. 19-24.
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