Impact of dietary aromatic amino acids on osteoclastic activity

Mona El Refaey, Qing Zhong, Kehong Ding, Xing Ming Shi, Jianrui Xu, Wendy B Bollag, William D Hill, Norman Chutkan, Richard Robbins, Hugh Nadeau, Maribeth H Johnson, Mark W Hamrick, Carlos M Isales

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We had shown that aromatic amino acid (phenylalanine, tyrosine, and tryptophan) supplementation prevented bone loss in an aging C57BL/6 mice model. In vivo results from the markers of bone breakdown suggested an inhibition of osteoclastic activity or differentiation. To assess osteoclastic differentiation, we examined the effects of aromatic amino acids on early /structural markers as vitronectin receptor, calcitonin receptor, and carbonic anhydrase II as well as, late/functional differentiation markers; cathepsin K and matrix metalloproteinase 9 (MMP-9). Our data demonstrate that the aromatic amino acids down-regulated early and late osteoclastic differentiation markers as measured by real time PCR. Our data also suggest a link between the vitronectin receptor and the secreted cathepsin K that both showed consistent effects to the aromatic amino acid treatment. However, the non-attachment related proteins, calcitonin receptor, and carbonic anhydrase II, demonstrated less consistent effects in response to treatment. Our data are consistent with aromatic amino acids down-regulating osteoclastic differentiation by suppressing remodeling gene expression thus contributing initially to the net increase in bone mass seen in vivo.

Original languageEnglish (US)
Pages (from-to)174-182
Number of pages9
JournalCalcified Tissue International
Volume95
Issue number2
DOIs
StatePublished - Jan 1 2014

Fingerprint

Aromatic Amino Acids
Calcitonin Receptors
Integrin alphaVbeta3
Cathepsin K
Carbonic Anhydrase II
Differentiation Antigens
Bone and Bones
Matrix Metalloproteinase 9
Phenylalanine
Inbred C57BL Mouse
Tryptophan
Tyrosine
Real-Time Polymerase Chain Reaction
Gene Expression
Proteins

Keywords

  • Amino acids
  • Calcitonin receptor
  • Carbonic anhydrase II
  • Cathepsin K
  • Osteoclast

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

Cite this

Impact of dietary aromatic amino acids on osteoclastic activity. / Refaey, Mona El; Zhong, Qing; Ding, Kehong; Shi, Xing Ming; Xu, Jianrui; Bollag, Wendy B; Hill, William D; Chutkan, Norman; Robbins, Richard; Nadeau, Hugh; Johnson, Maribeth H; Hamrick, Mark W; Isales, Carlos M.

In: Calcified Tissue International, Vol. 95, No. 2, 01.01.2014, p. 174-182.

Research output: Contribution to journalArticle

Refaey, Mona El ; Zhong, Qing ; Ding, Kehong ; Shi, Xing Ming ; Xu, Jianrui ; Bollag, Wendy B ; Hill, William D ; Chutkan, Norman ; Robbins, Richard ; Nadeau, Hugh ; Johnson, Maribeth H ; Hamrick, Mark W ; Isales, Carlos M. / Impact of dietary aromatic amino acids on osteoclastic activity. In: Calcified Tissue International. 2014 ; Vol. 95, No. 2. pp. 174-182.
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