Impaired E-cadherin expression in human spermatozoa in a male factor infertility subset signifies E-cadherin-mediated adhesion mechanisms operative in sperm-oolemma interactions

Sharad B Purohit, M. Brahmaraju, Abha Palta, Sunita Shukla, Malini Laloraya, Pradeep G. Kumar

Research output: Contribution to journalArticle

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Abstract

Cadherins comprise a family of calcium-dependent glycoproteins that function in mediating cell-cell adhesion in virtually all solid tissues of multicellular organisms. We have examined the presence of a cadherin on spermatozoon and its possible involvement in sperm-oocyte interaction. Spermatozoa from fertile human subjects showed the presence of E-cadherin on its head domains, detectable only after permeabilization of the surface membranes. On the contrary, spermatozoa from oligozoospermic subjects did not possess E-cadherin on their principal acrosomal and equatorial domains. Immunoprecipitation and Western blot studies also showed the presence of E-cadherin in spermatozoa from fertile males and its absence in oligozoospermic males. Using RT-PCR, we detected E-cadherin message in the round cells of fertile males, which was absent in the cells from oligozoospermic males. The presence of anti-E-cadherin antibody brought about quantitative reduction in the sperm-oocyte binding in vitro. These observations indicate the possibility of the interplay of a cadherin-dependent homophilic and/or heterophilic adhesion interaction between spermatozoa and oocyte during fertilization. The absence of a key adhesion molecule in a human male infertility disorder points towards genetic defects causing failure in gamete interactions.

Original languageEnglish (US)
Pages (from-to)903-909
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume316
Issue number3
DOIs
StatePublished - Apr 9 2004

Fingerprint

Male Infertility
Cadherins
Spermatozoa
Adhesion
Sperm-Ovum Interactions
Oocytes
Cell adhesion
Immunoprecipitation
Fertilization
Cell Adhesion
Glycoproteins
Western Blotting
Head
Tissue
Calcium
Membranes
Polymerase Chain Reaction
Defects
Molecules
Antibodies

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Impaired E-cadherin expression in human spermatozoa in a male factor infertility subset signifies E-cadherin-mediated adhesion mechanisms operative in sperm-oolemma interactions. / Purohit, Sharad B; Brahmaraju, M.; Palta, Abha; Shukla, Sunita; Laloraya, Malini; Kumar, Pradeep G.

In: Biochemical and Biophysical Research Communications, Vol. 316, No. 3, 09.04.2004, p. 903-909.

Research output: Contribution to journalArticle

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