Impaired NO-dependent dilation of skeletal muscle arterioles in hypertensive diabetic obese Zucker rats

J. C. Frisbee, David W Stepp

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

This study determined alterations to nitric oxide (NO)-dependent dilation of skeletal muscle arterioles from obese (OZR) versus lean Zucker rats (LZR). In situ cremaster muscle arterioles from both groups were viewed via television microscopy, and vessel dilation was measured with a video micrometer. Arteriolar dilation to acetylcholine and sodium nitroprusside was reduced in OZR versus LZR, although dilation to aprikalim was unaltered. NO-dependent flow-induced arteriolar dilation (via parallel microvessel occlusion) was attenuated in OZR, impairing arteriolar ability to regulate wall shear rate. Vascular superoxide levels, as assessed by dihydroethidine fluorescence, were elevated in OZR versus LZR. Treatment of cremaster muscles of OZR with the superoxide scavengers polyethylene glycol-superoxide dismutase and catalase improved arteriolar dilation to acetylcholine and sodium nitroprusside and restored flow-induced dilation and microvascular ability to regulate wall shear rate. These results suggest that NO-dependent dilation of skeletal muscle microvessels in OZR is impaired due to increased levels of superoxide. Taken together, these data suggest that the development of diabetes and hypertension in OZR may be associated with an impaired skeletal muscle perfusion via an elevated vascular oxidant stress.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume281
Issue number3 50-3
StatePublished - Oct 1 2001
Externally publishedYes

Fingerprint

Zucker Rats
Arterioles
Dilatation
Nitric Oxide
Skeletal Muscle
Superoxides
Abdominal Muscles
Nitroprusside
Microvessels
Acetylcholine
Blood Vessels
Television
Oxidants
Catalase
Microscopy
Perfusion
Fluorescence
Hypertension

Keywords

  • Flow-induced dilation
  • Hypertension
  • Skeletal muscle microcirculation
  • Superoxide
  • Type II diabetes

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

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