Impaired wound healing in hypoxic renal tubular cells: Roles of hypoxia-inducible factor-1 and glycogen synthase kinase 3β/β-catenin signaling

Jianping Peng, Ganesan Ramesh, Lin Sun, Zheng Dong

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Wound and subsequent healing are frequently associated with hypoxia. Although hypoxia induces angiogenesis for tissue remodeling during wound healing, it may also affect the healing response of parenchymal cells. Whether and how wound healing is affected by hypoxia in kidney cells and tissues is currently unknown. Here, we used scratch-wound healing and transwell migration models to examine the effect of hypoxia in cultured renal proximal tubular cells (RPTC). Wound healing and migration were significantly slower in hypoxic (1% oxygen) RPTC than normoxic (21% oxygen) cells. Hypoxia-inducible factor-1α(HIF-1α) was induced during scratch-wound healing in normoxia, and the induction was more evident in hypoxia. Nevertheless, HIF-1α-null and wild-type cells healed similarly after scratch wounding. Moreover, activation of HIF-1α with dimethyloxalylglycine in normoxic cells did not suppress wound healing, negating a major role of HIF-1α in wound healing in this model. Scratch-wound healing was also associated with glycogen synthase kinase 3β (GSK3β)/β-catenin signaling, which was further enhanced by hypoxia. Pharmacological inhibition of GSK3β resulted in β-catenin expression, accompanied by the suppression of wound healing and transwell cell migration. Ectopic expression of β-catenin in normoxic cells could also suppress wound healing, mimicking the effect of hypoxia. Conversely, inhibition of β-catenin via dominant negative mutants or short hairpin RNA improved wound healing and transwell migration in hypoxic cells. The results suggest that GSK3β/β- catenin signaling may contribute to defective wound healing in hypoxic renal cells and tissues.

Original languageEnglish (US)
Pages (from-to)176-184
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume340
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Glycogen Synthase Kinase 3
Hypoxia-Inducible Factor 1
Cell Hypoxia
Catenins
Wound Healing
Kidney
Oxygen
Null Lymphocytes
Hypoxia
Small Interfering RNA
Cell Movement

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Impaired wound healing in hypoxic renal tubular cells : Roles of hypoxia-inducible factor-1 and glycogen synthase kinase 3β/β-catenin signaling. / Peng, Jianping; Ramesh, Ganesan; Sun, Lin; Dong, Zheng.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 340, No. 1, 01.01.2012, p. 176-184.

Research output: Contribution to journalArticle

@article{9a7c7351fa864d33a7585551a3685cbb,
title = "Impaired wound healing in hypoxic renal tubular cells: Roles of hypoxia-inducible factor-1 and glycogen synthase kinase 3β/β-catenin signaling",
abstract = "Wound and subsequent healing are frequently associated with hypoxia. Although hypoxia induces angiogenesis for tissue remodeling during wound healing, it may also affect the healing response of parenchymal cells. Whether and how wound healing is affected by hypoxia in kidney cells and tissues is currently unknown. Here, we used scratch-wound healing and transwell migration models to examine the effect of hypoxia in cultured renal proximal tubular cells (RPTC). Wound healing and migration were significantly slower in hypoxic (1{\%} oxygen) RPTC than normoxic (21{\%} oxygen) cells. Hypoxia-inducible factor-1α(HIF-1α) was induced during scratch-wound healing in normoxia, and the induction was more evident in hypoxia. Nevertheless, HIF-1α-null and wild-type cells healed similarly after scratch wounding. Moreover, activation of HIF-1α with dimethyloxalylglycine in normoxic cells did not suppress wound healing, negating a major role of HIF-1α in wound healing in this model. Scratch-wound healing was also associated with glycogen synthase kinase 3β (GSK3β)/β-catenin signaling, which was further enhanced by hypoxia. Pharmacological inhibition of GSK3β resulted in β-catenin expression, accompanied by the suppression of wound healing and transwell cell migration. Ectopic expression of β-catenin in normoxic cells could also suppress wound healing, mimicking the effect of hypoxia. Conversely, inhibition of β-catenin via dominant negative mutants or short hairpin RNA improved wound healing and transwell migration in hypoxic cells. The results suggest that GSK3β/β- catenin signaling may contribute to defective wound healing in hypoxic renal cells and tissues.",
author = "Jianping Peng and Ganesan Ramesh and Lin Sun and Zheng Dong",
year = "2012",
month = "1",
day = "1",
doi = "10.1124/jpet.111.187427",
language = "English (US)",
volume = "340",
pages = "176--184",
journal = "The Journal of pharmacology and experimental therapeutics",
issn = "0022-3565",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "1",

}

TY - JOUR

T1 - Impaired wound healing in hypoxic renal tubular cells

T2 - Roles of hypoxia-inducible factor-1 and glycogen synthase kinase 3β/β-catenin signaling

AU - Peng, Jianping

AU - Ramesh, Ganesan

AU - Sun, Lin

AU - Dong, Zheng

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Wound and subsequent healing are frequently associated with hypoxia. Although hypoxia induces angiogenesis for tissue remodeling during wound healing, it may also affect the healing response of parenchymal cells. Whether and how wound healing is affected by hypoxia in kidney cells and tissues is currently unknown. Here, we used scratch-wound healing and transwell migration models to examine the effect of hypoxia in cultured renal proximal tubular cells (RPTC). Wound healing and migration were significantly slower in hypoxic (1% oxygen) RPTC than normoxic (21% oxygen) cells. Hypoxia-inducible factor-1α(HIF-1α) was induced during scratch-wound healing in normoxia, and the induction was more evident in hypoxia. Nevertheless, HIF-1α-null and wild-type cells healed similarly after scratch wounding. Moreover, activation of HIF-1α with dimethyloxalylglycine in normoxic cells did not suppress wound healing, negating a major role of HIF-1α in wound healing in this model. Scratch-wound healing was also associated with glycogen synthase kinase 3β (GSK3β)/β-catenin signaling, which was further enhanced by hypoxia. Pharmacological inhibition of GSK3β resulted in β-catenin expression, accompanied by the suppression of wound healing and transwell cell migration. Ectopic expression of β-catenin in normoxic cells could also suppress wound healing, mimicking the effect of hypoxia. Conversely, inhibition of β-catenin via dominant negative mutants or short hairpin RNA improved wound healing and transwell migration in hypoxic cells. The results suggest that GSK3β/β- catenin signaling may contribute to defective wound healing in hypoxic renal cells and tissues.

AB - Wound and subsequent healing are frequently associated with hypoxia. Although hypoxia induces angiogenesis for tissue remodeling during wound healing, it may also affect the healing response of parenchymal cells. Whether and how wound healing is affected by hypoxia in kidney cells and tissues is currently unknown. Here, we used scratch-wound healing and transwell migration models to examine the effect of hypoxia in cultured renal proximal tubular cells (RPTC). Wound healing and migration were significantly slower in hypoxic (1% oxygen) RPTC than normoxic (21% oxygen) cells. Hypoxia-inducible factor-1α(HIF-1α) was induced during scratch-wound healing in normoxia, and the induction was more evident in hypoxia. Nevertheless, HIF-1α-null and wild-type cells healed similarly after scratch wounding. Moreover, activation of HIF-1α with dimethyloxalylglycine in normoxic cells did not suppress wound healing, negating a major role of HIF-1α in wound healing in this model. Scratch-wound healing was also associated with glycogen synthase kinase 3β (GSK3β)/β-catenin signaling, which was further enhanced by hypoxia. Pharmacological inhibition of GSK3β resulted in β-catenin expression, accompanied by the suppression of wound healing and transwell cell migration. Ectopic expression of β-catenin in normoxic cells could also suppress wound healing, mimicking the effect of hypoxia. Conversely, inhibition of β-catenin via dominant negative mutants or short hairpin RNA improved wound healing and transwell migration in hypoxic cells. The results suggest that GSK3β/β- catenin signaling may contribute to defective wound healing in hypoxic renal cells and tissues.

UR - http://www.scopus.com/inward/record.url?scp=84055223413&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84055223413&partnerID=8YFLogxK

U2 - 10.1124/jpet.111.187427

DO - 10.1124/jpet.111.187427

M3 - Article

C2 - 22010210

AN - SCOPUS:84055223413

VL - 340

SP - 176

EP - 184

JO - The Journal of pharmacology and experimental therapeutics

JF - The Journal of pharmacology and experimental therapeutics

SN - 0022-3565

IS - 1

ER -