Imperfect hybrid layers created by an aggressive one-step self-etch adhesive in primary dentin are amendable to biomimetic remineralization in vitro

Jongryul Kim, Ryan M. Vaughn, Lisha Gu, Roy A. Rockman, Dwayne D. Arola, Tara E. Schafer, Kyoung Kyu Choi, David H. Pashley, Franklin R. Tay

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Degradation of hybrid layers created in primary dentin occurs as early as 6 months in vivo. Biomimetic remineralization utilizes "bottom-up" nanotechnology principles for interfibrillar and intrafibrillar remineralization of collagen matrices. This study examined whether imperfect hybrid layers created in primary dentin can be remineralized. Coronal dentin surfaces were prepared from extracted primary molars and bonded using Adper Prompt L-Pop and a composite. One-millimeter-thick specimen slabs of the resin-dentin interface were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs to mimic the sequestration and templating functions of dentin noncollagenous proteins. Specimens were retrieved after 1-6 months. Confocal laser scanning microscopy was used for evaluating the permeability of hybrid layers to Rhodamine B. Transmission electron microscopy was used to examine the status of remineralization within hybrid layers. Remineralization at different locations of the hybrid layers corresponded with quenching of fluorescence within similar locations of those hybrid layers. Remineralization was predominantly intrafibrillar in nature as interfibrillar spaces were filled with adhesive resin. Biomimetic remineralization of imperfect hybrid layers in primary human dentin is a potential means for preserving bond integrity. The success of the current proof-of-concept, laterally diffusing remineralization protocol warrants development of a clinically applicable biomimetic remineralization delivery system.

Original languageEnglish (US)
Pages (from-to)1225-1234
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume93
Issue number4
DOIs
StatePublished - Jun 15 2010

Fingerprint

Biomimetics
Dentin
Adhesives
rhodamine B
Resins
Portland cement
Nanotechnology
Collagen
Quenching
Microscopic examination
Fluorescence
Transmission electron microscopy
Proteins
Scanning
Transmission Electron Microscopy
Confocal Microscopy
Degradation
In Vitro Techniques
Lasers
Permeability

Keywords

  • Biomimetic remineralization
  • Interfibrillar
  • Intrafibrillar
  • One-step adhesive
  • Primary dentin
  • Self-etching

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Imperfect hybrid layers created by an aggressive one-step self-etch adhesive in primary dentin are amendable to biomimetic remineralization in vitro. / Kim, Jongryul; Vaughn, Ryan M.; Gu, Lisha; Rockman, Roy A.; Arola, Dwayne D.; Schafer, Tara E.; Choi, Kyoung Kyu; Pashley, David H.; Tay, Franklin R.

In: Journal of Biomedical Materials Research - Part A, Vol. 93, No. 4, 15.06.2010, p. 1225-1234.

Research output: Contribution to journalArticle

@article{10ea11b34d214385b6aedcf97c0460d2,
title = "Imperfect hybrid layers created by an aggressive one-step self-etch adhesive in primary dentin are amendable to biomimetic remineralization in vitro",
abstract = "Degradation of hybrid layers created in primary dentin occurs as early as 6 months in vivo. Biomimetic remineralization utilizes {"}bottom-up{"} nanotechnology principles for interfibrillar and intrafibrillar remineralization of collagen matrices. This study examined whether imperfect hybrid layers created in primary dentin can be remineralized. Coronal dentin surfaces were prepared from extracted primary molars and bonded using Adper Prompt L-Pop and a composite. One-millimeter-thick specimen slabs of the resin-dentin interface were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs to mimic the sequestration and templating functions of dentin noncollagenous proteins. Specimens were retrieved after 1-6 months. Confocal laser scanning microscopy was used for evaluating the permeability of hybrid layers to Rhodamine B. Transmission electron microscopy was used to examine the status of remineralization within hybrid layers. Remineralization at different locations of the hybrid layers corresponded with quenching of fluorescence within similar locations of those hybrid layers. Remineralization was predominantly intrafibrillar in nature as interfibrillar spaces were filled with adhesive resin. Biomimetic remineralization of imperfect hybrid layers in primary human dentin is a potential means for preserving bond integrity. The success of the current proof-of-concept, laterally diffusing remineralization protocol warrants development of a clinically applicable biomimetic remineralization delivery system.",
keywords = "Biomimetic remineralization, Interfibrillar, Intrafibrillar, One-step adhesive, Primary dentin, Self-etching",
author = "Jongryul Kim and Vaughn, {Ryan M.} and Lisha Gu and Rockman, {Roy A.} and Arola, {Dwayne D.} and Schafer, {Tara E.} and Choi, {Kyoung Kyu} and Pashley, {David H.} and Tay, {Franklin R.}",
year = "2010",
month = "6",
day = "15",
doi = "10.1002/jbm.a.32612",
language = "English (US)",
volume = "93",
pages = "1225--1234",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "0021-9304",
publisher = "Heterocorporation",
number = "4",

}

TY - JOUR

T1 - Imperfect hybrid layers created by an aggressive one-step self-etch adhesive in primary dentin are amendable to biomimetic remineralization in vitro

AU - Kim, Jongryul

AU - Vaughn, Ryan M.

AU - Gu, Lisha

AU - Rockman, Roy A.

AU - Arola, Dwayne D.

AU - Schafer, Tara E.

AU - Choi, Kyoung Kyu

AU - Pashley, David H.

AU - Tay, Franklin R.

PY - 2010/6/15

Y1 - 2010/6/15

N2 - Degradation of hybrid layers created in primary dentin occurs as early as 6 months in vivo. Biomimetic remineralization utilizes "bottom-up" nanotechnology principles for interfibrillar and intrafibrillar remineralization of collagen matrices. This study examined whether imperfect hybrid layers created in primary dentin can be remineralized. Coronal dentin surfaces were prepared from extracted primary molars and bonded using Adper Prompt L-Pop and a composite. One-millimeter-thick specimen slabs of the resin-dentin interface were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs to mimic the sequestration and templating functions of dentin noncollagenous proteins. Specimens were retrieved after 1-6 months. Confocal laser scanning microscopy was used for evaluating the permeability of hybrid layers to Rhodamine B. Transmission electron microscopy was used to examine the status of remineralization within hybrid layers. Remineralization at different locations of the hybrid layers corresponded with quenching of fluorescence within similar locations of those hybrid layers. Remineralization was predominantly intrafibrillar in nature as interfibrillar spaces were filled with adhesive resin. Biomimetic remineralization of imperfect hybrid layers in primary human dentin is a potential means for preserving bond integrity. The success of the current proof-of-concept, laterally diffusing remineralization protocol warrants development of a clinically applicable biomimetic remineralization delivery system.

AB - Degradation of hybrid layers created in primary dentin occurs as early as 6 months in vivo. Biomimetic remineralization utilizes "bottom-up" nanotechnology principles for interfibrillar and intrafibrillar remineralization of collagen matrices. This study examined whether imperfect hybrid layers created in primary dentin can be remineralized. Coronal dentin surfaces were prepared from extracted primary molars and bonded using Adper Prompt L-Pop and a composite. One-millimeter-thick specimen slabs of the resin-dentin interface were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs to mimic the sequestration and templating functions of dentin noncollagenous proteins. Specimens were retrieved after 1-6 months. Confocal laser scanning microscopy was used for evaluating the permeability of hybrid layers to Rhodamine B. Transmission electron microscopy was used to examine the status of remineralization within hybrid layers. Remineralization at different locations of the hybrid layers corresponded with quenching of fluorescence within similar locations of those hybrid layers. Remineralization was predominantly intrafibrillar in nature as interfibrillar spaces were filled with adhesive resin. Biomimetic remineralization of imperfect hybrid layers in primary human dentin is a potential means for preserving bond integrity. The success of the current proof-of-concept, laterally diffusing remineralization protocol warrants development of a clinically applicable biomimetic remineralization delivery system.

KW - Biomimetic remineralization

KW - Interfibrillar

KW - Intrafibrillar

KW - One-step adhesive

KW - Primary dentin

KW - Self-etching

UR - http://www.scopus.com/inward/record.url?scp=77951573365&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951573365&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.32612

DO - 10.1002/jbm.a.32612

M3 - Article

C2 - 19768792

AN - SCOPUS:77951573365

VL - 93

SP - 1225

EP - 1234

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 0021-9304

IS - 4

ER -