Imperfect hybrid layers created by an aggressive one-step self-etch adhesive in primary dentin are amendable to biomimetic remineralization in vitro

Jongryul Kim, Ryan M. Vaughn, Lisha Gu, Roy A. Rockman, Dwayne D. Arola, Tara E. Schafer, Kyoung Kyu Choi, David H. Pashley, Franklin R. Tay

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Degradation of hybrid layers created in primary dentin occurs as early as 6 months in vivo. Biomimetic remineralization utilizes "bottom-up" nanotechnology principles for interfibrillar and intrafibrillar remineralization of collagen matrices. This study examined whether imperfect hybrid layers created in primary dentin can be remineralized. Coronal dentin surfaces were prepared from extracted primary molars and bonded using Adper Prompt L-Pop and a composite. One-millimeter-thick specimen slabs of the resin-dentin interface were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs to mimic the sequestration and templating functions of dentin noncollagenous proteins. Specimens were retrieved after 1-6 months. Confocal laser scanning microscopy was used for evaluating the permeability of hybrid layers to Rhodamine B. Transmission electron microscopy was used to examine the status of remineralization within hybrid layers. Remineralization at different locations of the hybrid layers corresponded with quenching of fluorescence within similar locations of those hybrid layers. Remineralization was predominantly intrafibrillar in nature as interfibrillar spaces were filled with adhesive resin. Biomimetic remineralization of imperfect hybrid layers in primary human dentin is a potential means for preserving bond integrity. The success of the current proof-of-concept, laterally diffusing remineralization protocol warrants development of a clinically applicable biomimetic remineralization delivery system.

Original languageEnglish (US)
Pages (from-to)1225-1234
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume93
Issue number4
DOIs
StatePublished - Jun 15 2010

Keywords

  • Biomimetic remineralization
  • Interfibrillar
  • Intrafibrillar
  • One-step adhesive
  • Primary dentin
  • Self-etching

ASJC Scopus subject areas

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

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