Implication of ethanol wet-bonding in hybrid layer remineralization

J. Kim, L. Gu, L. Breschi, L. Tjäderhane, K. K. Choi, David Henry Pashley, Franklin Chi Meng Tay

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

During mineralization, unbound water within the collagen matrix is replaced by apatite. This study tested the null hypothesis that there is no difference in the status of in vitro biomimetic remineralization of hybrid layers, regardless of their moisture contents. Acid-etched dentin was bonded with One-Step with ethanol-wet-bonding, water-wet-bonding, and water-overwet-bonding protocols. Composite-dentin slabs were subjected to remineralization for 1-4 months in a medium containing dual biomimetic analogs, with set Portland cement as the calcium source and characterized by transmission electron microscopy. Remineralization was either non-existent or restricted to the intrafibrillar mode in ethanol-wet-bonded specimens. Extensive intrafibrillar and interfibrillar remineralization was observed in water-wet-bonded specimens. Water-overwet specimens demonstrated partial remineralization of hybrid layers and precipitation of mineralized plates within water channels. The use of ethanol-wet-bonding substantiates that biomimetic remineralization is a progressive dehydration process that replaces residual water in hybrid layers with apatite crystallites.

Original languageEnglish (US)
Pages (from-to)575-580
Number of pages6
JournalJournal of Dental Research
Volume89
Issue number6
DOIs
StatePublished - Jun 1 2010

Fingerprint

Ethanol
Biomimetics
Water
Apatites
Dentin
Aquaporins
Transmission Electron Microscopy
Dehydration
Collagen
Calcium
Acids

Keywords

  • Biomimetics
  • Dentin bonding
  • Ethanol
  • Hybrid layer
  • Interfibrillar
  • Intrafibrillar
  • Remineralization
  • Water

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Kim, J., Gu, L., Breschi, L., Tjäderhane, L., Choi, K. K., Pashley, D. H., & Tay, F. C. M. (2010). Implication of ethanol wet-bonding in hybrid layer remineralization. Journal of Dental Research, 89(6), 575-580. https://doi.org/10.1177/0022034510363380

Implication of ethanol wet-bonding in hybrid layer remineralization. / Kim, J.; Gu, L.; Breschi, L.; Tjäderhane, L.; Choi, K. K.; Pashley, David Henry; Tay, Franklin Chi Meng.

In: Journal of Dental Research, Vol. 89, No. 6, 01.06.2010, p. 575-580.

Research output: Contribution to journalArticle

Kim, J, Gu, L, Breschi, L, Tjäderhane, L, Choi, KK, Pashley, DH & Tay, FCM 2010, 'Implication of ethanol wet-bonding in hybrid layer remineralization', Journal of Dental Research, vol. 89, no. 6, pp. 575-580. https://doi.org/10.1177/0022034510363380
Kim J, Gu L, Breschi L, Tjäderhane L, Choi KK, Pashley DH et al. Implication of ethanol wet-bonding in hybrid layer remineralization. Journal of Dental Research. 2010 Jun 1;89(6):575-580. https://doi.org/10.1177/0022034510363380
Kim, J. ; Gu, L. ; Breschi, L. ; Tjäderhane, L. ; Choi, K. K. ; Pashley, David Henry ; Tay, Franklin Chi Meng. / Implication of ethanol wet-bonding in hybrid layer remineralization. In: Journal of Dental Research. 2010 ; Vol. 89, No. 6. pp. 575-580.
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