Improved immunohistochemical detection of p53 protein in paraffin - embedded tissues reveals elevated levels in most head and neck and lung carcinomas

Correlation with clinicopathological parameters

Z. P. Pavelic, J. L. Gluckman, M. Gapany, J. Reising, J. M. Craven, D. J. Kelley, L. Pavelic, S. Gapany, Paul Williams Biddinger, P. J. Stambrook

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

We have analyzed the expression of the p53 tumor suppressor gene in paraffin-embedded sections of normal and malignant head and neck and lung tumors by immunohistochemistry using the PAb 1801 monoclonal antibody (MAb). The PAb 1801 does not consistently detect its p53 epitope in tissue fixed in 10% buffered formaldehyde. However, the antibody is effective in AMeX-fixed specimens, thereby permitting the improved morphologic localization of p53 phosphoprotein in paraffin embedded tissue. Of 33 primary head and neck carcinomas analyzed from AMeX-fixed, paraffin-embedded sections, 21 (64%) showed heterogeneous staining with PAb 1801. All 33 normal samples of head and neck tissues were negative. Similarly, 13 out of 20 lung carcinomas (65%) showed heterogeneous staining while none of normal lung tissues were positive. The data indicate a strong positive correlation between p53 detection by PAb 1801 and carcinomas of the head and neck and of lung. However, there was no obvious correlation between p53 staining and the number of involved nodes, the stage of disease or the degree of differentiation in these carcinomas.

Original languageEnglish (US)
Pages (from-to)1389-1394
Number of pages6
JournalAnticancer research
Volume12
Issue number5
StatePublished - Jan 1 1992
Externally publishedYes

Fingerprint

Paraffin
Neck
Head
Carcinoma
Lung
Staining and Labeling
Proteins
Phosphoproteins
Tumor Suppressor Genes
Formaldehyde
Epitopes
Immunohistochemistry
Monoclonal Antibodies
Antibodies
Neoplasms

Keywords

  • Head and neck cancer
  • Immunohistochemistry
  • Lung cancer
  • Paraffin embedded tissues
  • p53 suppressor gene

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Improved immunohistochemical detection of p53 protein in paraffin - embedded tissues reveals elevated levels in most head and neck and lung carcinomas : Correlation with clinicopathological parameters. / Pavelic, Z. P.; Gluckman, J. L.; Gapany, M.; Reising, J.; Craven, J. M.; Kelley, D. J.; Pavelic, L.; Gapany, S.; Biddinger, Paul Williams; Stambrook, P. J.

In: Anticancer research, Vol. 12, No. 5, 01.01.1992, p. 1389-1394.

Research output: Contribution to journalArticle

Pavelic, Z. P. ; Gluckman, J. L. ; Gapany, M. ; Reising, J. ; Craven, J. M. ; Kelley, D. J. ; Pavelic, L. ; Gapany, S. ; Biddinger, Paul Williams ; Stambrook, P. J. / Improved immunohistochemical detection of p53 protein in paraffin - embedded tissues reveals elevated levels in most head and neck and lung carcinomas : Correlation with clinicopathological parameters. In: Anticancer research. 1992 ; Vol. 12, No. 5. pp. 1389-1394.
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abstract = "We have analyzed the expression of the p53 tumor suppressor gene in paraffin-embedded sections of normal and malignant head and neck and lung tumors by immunohistochemistry using the PAb 1801 monoclonal antibody (MAb). The PAb 1801 does not consistently detect its p53 epitope in tissue fixed in 10{\%} buffered formaldehyde. However, the antibody is effective in AMeX-fixed specimens, thereby permitting the improved morphologic localization of p53 phosphoprotein in paraffin embedded tissue. Of 33 primary head and neck carcinomas analyzed from AMeX-fixed, paraffin-embedded sections, 21 (64{\%}) showed heterogeneous staining with PAb 1801. All 33 normal samples of head and neck tissues were negative. Similarly, 13 out of 20 lung carcinomas (65{\%}) showed heterogeneous staining while none of normal lung tissues were positive. The data indicate a strong positive correlation between p53 detection by PAb 1801 and carcinomas of the head and neck and of lung. However, there was no obvious correlation between p53 staining and the number of involved nodes, the stage of disease or the degree of differentiation in these carcinomas.",
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AB - We have analyzed the expression of the p53 tumor suppressor gene in paraffin-embedded sections of normal and malignant head and neck and lung tumors by immunohistochemistry using the PAb 1801 monoclonal antibody (MAb). The PAb 1801 does not consistently detect its p53 epitope in tissue fixed in 10% buffered formaldehyde. However, the antibody is effective in AMeX-fixed specimens, thereby permitting the improved morphologic localization of p53 phosphoprotein in paraffin embedded tissue. Of 33 primary head and neck carcinomas analyzed from AMeX-fixed, paraffin-embedded sections, 21 (64%) showed heterogeneous staining with PAb 1801. All 33 normal samples of head and neck tissues were negative. Similarly, 13 out of 20 lung carcinomas (65%) showed heterogeneous staining while none of normal lung tissues were positive. The data indicate a strong positive correlation between p53 detection by PAb 1801 and carcinomas of the head and neck and of lung. However, there was no obvious correlation between p53 staining and the number of involved nodes, the stage of disease or the degree of differentiation in these carcinomas.

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