Improvement in relaxation response in corpus cavernosum from trained rats

Mário A. Claudino, Fernanda Priviero, Cleber E. Teixeira, Gilberto De Nucci, Edson Antunes, Angelina Zanesco

Research output: Contribution to journalArticle

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Abstract

Objectives To evaluate the contractile and relaxing responses in rat corpus cavernosum (RCC) from rats after 8 weeks of run training, because erectile function is highly dependent on nitric oxide (NO) from nitrergic fibers or endothelium. Physical activity enhances NO production and improves endothelial function, with beneficial effects on cardiovascular disease. Methods The training program consisted of 8 weeks of run training, 5 days/wk, and each session lasted 60 minutes. The RCC was isolated, and concentration-response curves to NO, acetylcholine, sodium nitroprusside, phenylephrine, and endothelin were obtained. The excitatory and inhibitory effects of electrical field stimulation (2 to 32 Hz) were also evaluated. Results NO (0.1 to 100 μM) and sodium nitroprusside (0.01 to 1000 μM) produced a relaxing effect in RCC in a dose-dependent manner, with the maximal responses to NO (control 62% ± 4%, trained 88% ± 3%) and sodium nitroprusside (control 83% ± 3%, trained 95% ± 2%) significantly enhanced after 8 weeks of run training. However, acetylcholine-induced relaxations were not affected by exercise. Similarly, electrical field stimulation-induced relaxations were significantly increased in RCC from trained rats at 2 Hz (control 2.4% ± 0.3%, trained 4.2% ± 0.5%) and 4 Hz (control 5.3% ± 1.2%, trained 12.5% ± 1.7%). The contractile sensitivity of RCC to phenylephrine (0.01 to 100 μM) and endothelin (0.01 to 100 nM) was not modified by training exercise. Conclusions Our findings suggest that run training enhances functional responses in rat RCC that involves increases in the NO-cyclic guanosine monophosphate signaling pathway by endothelium-independent mechanisms that is not accompanied by changes in contractile sensitivity.

Original languageEnglish (US)
Pages (from-to)1004-1008
Number of pages5
JournalUrology
Volume63
Issue number5
DOIs
StatePublished - May 1 2004

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Nitric Oxide
Nitroprusside
Endothelins
Phenylephrine
Electric Stimulation
Acetylcholine
Endothelium
Cyclic GMP
Cardiovascular Diseases
Exercise
Education

ASJC Scopus subject areas

  • Urology

Cite this

Claudino, M. A., Priviero, F., Teixeira, C. E., De Nucci, G., Antunes, E., & Zanesco, A. (2004). Improvement in relaxation response in corpus cavernosum from trained rats. Urology, 63(5), 1004-1008. https://doi.org/10.1016/j.urology.2003.11.034

Improvement in relaxation response in corpus cavernosum from trained rats. / Claudino, Mário A.; Priviero, Fernanda; Teixeira, Cleber E.; De Nucci, Gilberto; Antunes, Edson; Zanesco, Angelina.

In: Urology, Vol. 63, No. 5, 01.05.2004, p. 1004-1008.

Research output: Contribution to journalArticle

Claudino, MA, Priviero, F, Teixeira, CE, De Nucci, G, Antunes, E & Zanesco, A 2004, 'Improvement in relaxation response in corpus cavernosum from trained rats', Urology, vol. 63, no. 5, pp. 1004-1008. https://doi.org/10.1016/j.urology.2003.11.034
Claudino MA, Priviero F, Teixeira CE, De Nucci G, Antunes E, Zanesco A. Improvement in relaxation response in corpus cavernosum from trained rats. Urology. 2004 May 1;63(5):1004-1008. https://doi.org/10.1016/j.urology.2003.11.034
Claudino, Mário A. ; Priviero, Fernanda ; Teixeira, Cleber E. ; De Nucci, Gilberto ; Antunes, Edson ; Zanesco, Angelina. / Improvement in relaxation response in corpus cavernosum from trained rats. In: Urology. 2004 ; Vol. 63, No. 5. pp. 1004-1008.
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abstract = "Objectives To evaluate the contractile and relaxing responses in rat corpus cavernosum (RCC) from rats after 8 weeks of run training, because erectile function is highly dependent on nitric oxide (NO) from nitrergic fibers or endothelium. Physical activity enhances NO production and improves endothelial function, with beneficial effects on cardiovascular disease. Methods The training program consisted of 8 weeks of run training, 5 days/wk, and each session lasted 60 minutes. The RCC was isolated, and concentration-response curves to NO, acetylcholine, sodium nitroprusside, phenylephrine, and endothelin were obtained. The excitatory and inhibitory effects of electrical field stimulation (2 to 32 Hz) were also evaluated. Results NO (0.1 to 100 μM) and sodium nitroprusside (0.01 to 1000 μM) produced a relaxing effect in RCC in a dose-dependent manner, with the maximal responses to NO (control 62{\%} ± 4{\%}, trained 88{\%} ± 3{\%}) and sodium nitroprusside (control 83{\%} ± 3{\%}, trained 95{\%} ± 2{\%}) significantly enhanced after 8 weeks of run training. However, acetylcholine-induced relaxations were not affected by exercise. Similarly, electrical field stimulation-induced relaxations were significantly increased in RCC from trained rats at 2 Hz (control 2.4{\%} ± 0.3{\%}, trained 4.2{\%} ± 0.5{\%}) and 4 Hz (control 5.3{\%} ± 1.2{\%}, trained 12.5{\%} ± 1.7{\%}). The contractile sensitivity of RCC to phenylephrine (0.01 to 100 μM) and endothelin (0.01 to 100 nM) was not modified by training exercise. Conclusions Our findings suggest that run training enhances functional responses in rat RCC that involves increases in the NO-cyclic guanosine monophosphate signaling pathway by endothelium-independent mechanisms that is not accompanied by changes in contractile sensitivity.",
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N2 - Objectives To evaluate the contractile and relaxing responses in rat corpus cavernosum (RCC) from rats after 8 weeks of run training, because erectile function is highly dependent on nitric oxide (NO) from nitrergic fibers or endothelium. Physical activity enhances NO production and improves endothelial function, with beneficial effects on cardiovascular disease. Methods The training program consisted of 8 weeks of run training, 5 days/wk, and each session lasted 60 minutes. The RCC was isolated, and concentration-response curves to NO, acetylcholine, sodium nitroprusside, phenylephrine, and endothelin were obtained. The excitatory and inhibitory effects of electrical field stimulation (2 to 32 Hz) were also evaluated. Results NO (0.1 to 100 μM) and sodium nitroprusside (0.01 to 1000 μM) produced a relaxing effect in RCC in a dose-dependent manner, with the maximal responses to NO (control 62% ± 4%, trained 88% ± 3%) and sodium nitroprusside (control 83% ± 3%, trained 95% ± 2%) significantly enhanced after 8 weeks of run training. However, acetylcholine-induced relaxations were not affected by exercise. Similarly, electrical field stimulation-induced relaxations were significantly increased in RCC from trained rats at 2 Hz (control 2.4% ± 0.3%, trained 4.2% ± 0.5%) and 4 Hz (control 5.3% ± 1.2%, trained 12.5% ± 1.7%). The contractile sensitivity of RCC to phenylephrine (0.01 to 100 μM) and endothelin (0.01 to 100 nM) was not modified by training exercise. Conclusions Our findings suggest that run training enhances functional responses in rat RCC that involves increases in the NO-cyclic guanosine monophosphate signaling pathway by endothelium-independent mechanisms that is not accompanied by changes in contractile sensitivity.

AB - Objectives To evaluate the contractile and relaxing responses in rat corpus cavernosum (RCC) from rats after 8 weeks of run training, because erectile function is highly dependent on nitric oxide (NO) from nitrergic fibers or endothelium. Physical activity enhances NO production and improves endothelial function, with beneficial effects on cardiovascular disease. Methods The training program consisted of 8 weeks of run training, 5 days/wk, and each session lasted 60 minutes. The RCC was isolated, and concentration-response curves to NO, acetylcholine, sodium nitroprusside, phenylephrine, and endothelin were obtained. The excitatory and inhibitory effects of electrical field stimulation (2 to 32 Hz) were also evaluated. Results NO (0.1 to 100 μM) and sodium nitroprusside (0.01 to 1000 μM) produced a relaxing effect in RCC in a dose-dependent manner, with the maximal responses to NO (control 62% ± 4%, trained 88% ± 3%) and sodium nitroprusside (control 83% ± 3%, trained 95% ± 2%) significantly enhanced after 8 weeks of run training. However, acetylcholine-induced relaxations were not affected by exercise. Similarly, electrical field stimulation-induced relaxations were significantly increased in RCC from trained rats at 2 Hz (control 2.4% ± 0.3%, trained 4.2% ± 0.5%) and 4 Hz (control 5.3% ± 1.2%, trained 12.5% ± 1.7%). The contractile sensitivity of RCC to phenylephrine (0.01 to 100 μM) and endothelin (0.01 to 100 nM) was not modified by training exercise. Conclusions Our findings suggest that run training enhances functional responses in rat RCC that involves increases in the NO-cyclic guanosine monophosphate signaling pathway by endothelium-independent mechanisms that is not accompanied by changes in contractile sensitivity.

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