Improving heterologous gene expression in Aspergillus niger by introducing multiple copies of protein-binding sequence containing CCAAT to the promoter

L. Liu, J. Liu, R. X. Qiu, Xingguo Zhu, Z. Y. Dong, G. M. Tang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aims: To increase the expression level of heterologous gene in Aspergillus niger. Methods and Results: Inserting multiple copies of an activator protein-binding site originally encoded in the cis-regulatory region of A. niger glucoamylase gene (glaA), CCAAT-containing sequence, into the promoter of an expression plasmid greatly enhanced the production of heterologous protein. Conclusion: The promoter activity was increased by the introduction of the CCAAT-containing sequence resulting in increased gene expression. Significance and Impact of the Study: This study provides evidence that the CCAAT-containing region of the glaA promoter of A. niger is a binding site for a positive transcriptional factor and demonstrates the possibility for improving the expression of both heterologous and endogenous genes in Aspergillus.

Original languageEnglish (US)
Pages (from-to)358-361
Number of pages4
JournalLetters in Applied Microbiology
Volume36
Issue number6
DOIs
StatePublished - Jun 12 2003
Externally publishedYes

Fingerprint

Aspergillus niger
Protein Binding
Gene Expression
Binding Sites
Genes
Glucan 1,4-alpha-Glucosidase
Nucleic Acid Regulatory Sequences
Aspergillus
Genetic Promoter Regions
Plasmids
Proteins

Keywords

  • Aspergillus niger
  • CCAAT
  • Gene expression
  • Glucoamylase promoter

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Improving heterologous gene expression in Aspergillus niger by introducing multiple copies of protein-binding sequence containing CCAAT to the promoter. / Liu, L.; Liu, J.; Qiu, R. X.; Zhu, Xingguo; Dong, Z. Y.; Tang, G. M.

In: Letters in Applied Microbiology, Vol. 36, No. 6, 12.06.2003, p. 358-361.

Research output: Contribution to journalArticle

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AU - Liu, J.

AU - Qiu, R. X.

AU - Zhu, Xingguo

AU - Dong, Z. Y.

AU - Tang, G. M.

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