Abstract
An immunological method for the determination of the basic carbohydrate structure of gangliosides by using a thin-layer chromatographic immunostaining technique was developed. After high-performance thin-layer chromatography of gangliosides, the chromatogram is treated with a 0.4% polyisobutylmethacrylate solution. Arthrobacter ureafaciens neuraminidase is then applied to the separated gangliosides in situ on the chromatographic plate. This procedure will remove both external and internal sialic acid residues from the core oligosaccharide backbone. The resulting glycolipid products are then incubated with anti-Gg4 serum and 125I-staphylococcal protein A, successively, and exposed to an X-ray film. Through a highly specific binding, the anti-Gg4 antibody detects only those gangliosides having the oligosaccharide backbone of Gg4.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 54-58 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 148 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1985 |
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ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
Cite this
In situ immunological determination of basic carbohydrate structures of gangliosides on thin-layer plates. / Saito, Megumi; Kasai, Noriyuki; Yu, Robert K.
In: Analytical Biochemistry, Vol. 148, No. 1, 07.1985, p. 54-58.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In situ immunological determination of basic carbohydrate structures of gangliosides on thin-layer plates
AU - Saito, Megumi
AU - Kasai, Noriyuki
AU - Yu, Robert K.
PY - 1985/7
Y1 - 1985/7
N2 - An immunological method for the determination of the basic carbohydrate structure of gangliosides by using a thin-layer chromatographic immunostaining technique was developed. After high-performance thin-layer chromatography of gangliosides, the chromatogram is treated with a 0.4% polyisobutylmethacrylate solution. Arthrobacter ureafaciens neuraminidase is then applied to the separated gangliosides in situ on the chromatographic plate. This procedure will remove both external and internal sialic acid residues from the core oligosaccharide backbone. The resulting glycolipid products are then incubated with anti-Gg4 serum and 125I-staphylococcal protein A, successively, and exposed to an X-ray film. Through a highly specific binding, the anti-Gg4 antibody detects only those gangliosides having the oligosaccharide backbone of Gg4.
AB - An immunological method for the determination of the basic carbohydrate structure of gangliosides by using a thin-layer chromatographic immunostaining technique was developed. After high-performance thin-layer chromatography of gangliosides, the chromatogram is treated with a 0.4% polyisobutylmethacrylate solution. Arthrobacter ureafaciens neuraminidase is then applied to the separated gangliosides in situ on the chromatographic plate. This procedure will remove both external and internal sialic acid residues from the core oligosaccharide backbone. The resulting glycolipid products are then incubated with anti-Gg4 serum and 125I-staphylococcal protein A, successively, and exposed to an X-ray film. Through a highly specific binding, the anti-Gg4 antibody detects only those gangliosides having the oligosaccharide backbone of Gg4.
UR - http://www.scopus.com/inward/record.url?scp=0021845989&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021845989&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(85)90627-X
DO - 10.1016/0003-2697(85)90627-X
M3 - Article
C2 - 4037308
AN - SCOPUS:0021845989
VL - 148
SP - 54
EP - 58
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 1
ER -