In vitro antimicrobial activity of natural toxins and animal venoms tested against Burkholderia pseudomallei

R. Perumal Samy, Pachiappan Arjunan, P. Gopalakrishnakone, Maung M. Thwin, Yap E. Hian, Vincent T.K. Chow, Ho Bow, Joseph T. Weng

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Background: Burkholderia pseudomallei are the causative agent of melioidosis. Increasing resistance of the disease to antibiotics is a severe problem in treatment regime and has led to intensification of the search for new drugs. Antimicrobial peptides are the most ubiquitous in nature as part of the innate immune system and host defense mechanism. Methods: Here, we investigated a group of venoms (snakes, scorpions and honey bee venoms) for antimicrobial properties against two strains of Gram-negative bacteria Burkholderia pseudomallei by using disc-diffusion assay for in vitro susceptibility testing. The antibacterial activities of the venoms were compared with that of the isolated L-amino acid oxidase (LAAO) and phospholipase A2 (PLA2s) enzymes. MICs were determined using broth dilution method. Bacterial growth was assessed by measurement of optical density at the lowest dilutions (MIC 0.25 mg/ml). The cell viability was measured using tetrazolium salts (XTT) based cytotoxic assay. Results: The studied venoms showed high antimicrobial activity. The venoms of C. adamanteus, Daboia russelli russelli, A. halys, P. australis, B. candidus and P. guttata were equally as effective as Chloramphenicol and Ceftazidime (30 μg/disc). Among those tested, phospholipase A2 enzymes (crotoxin B and daboiatoxin) showed the most potent antibacterial activity against Gram-negative (TES) bacteria. Naturally occurring venom peptides and phospholipase A2 proved to possess highly potent antimicrobial activity against Burkholderia pseudomallei. The XTT-assay results showed that the cell survival decreased with increasing concentrations (0.05-10 mg/mL) of Crotalus adamanteus venom, with no effect on the cell viability evident at 0.5 mg/mL. Conclusion: This antibacterial profile of snake venoms reported herein will be useful in the search for potential antibacterial agents against drug resistant microorganisms like B. pseudomallei.

Original languageEnglish (US)
Article number100
JournalBMC Infectious Diseases
Volume6
DOIs
StatePublished - Jun 20 2006
Externally publishedYes

Fingerprint

Burkholderia pseudomallei
Venoms
Phospholipases A2
Cell Survival
Snake Venoms
Gram-Negative Bacteria
Crotoxin
L-Amino Acid Oxidase
Tetrazolium Salts
Melioidosis
Scorpion Venoms
Crotalus
Bee Venoms
Anti-Bacterial Agents
Peptides
Disease Resistance
Ceftazidime
Honey
Chloramphenicol
Enzymes

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Perumal Samy, R., Arjunan, P., Gopalakrishnakone, P., Thwin, M. M., Hian, Y. E., Chow, V. T. K., ... Weng, J. T. (2006). In vitro antimicrobial activity of natural toxins and animal venoms tested against Burkholderia pseudomallei. BMC Infectious Diseases, 6, [100]. https://doi.org/10.1186/1471-2334-6-100

In vitro antimicrobial activity of natural toxins and animal venoms tested against Burkholderia pseudomallei. / Perumal Samy, R.; Arjunan, Pachiappan; Gopalakrishnakone, P.; Thwin, Maung M.; Hian, Yap E.; Chow, Vincent T.K.; Bow, Ho; Weng, Joseph T.

In: BMC Infectious Diseases, Vol. 6, 100, 20.06.2006.

Research output: Contribution to journalArticle

Perumal Samy, R. ; Arjunan, Pachiappan ; Gopalakrishnakone, P. ; Thwin, Maung M. ; Hian, Yap E. ; Chow, Vincent T.K. ; Bow, Ho ; Weng, Joseph T. / In vitro antimicrobial activity of natural toxins and animal venoms tested against Burkholderia pseudomallei. In: BMC Infectious Diseases. 2006 ; Vol. 6.
@article{38d539d2a85a4ea1aa9bafc8077fc59c,
title = "In vitro antimicrobial activity of natural toxins and animal venoms tested against Burkholderia pseudomallei",
abstract = "Background: Burkholderia pseudomallei are the causative agent of melioidosis. Increasing resistance of the disease to antibiotics is a severe problem in treatment regime and has led to intensification of the search for new drugs. Antimicrobial peptides are the most ubiquitous in nature as part of the innate immune system and host defense mechanism. Methods: Here, we investigated a group of venoms (snakes, scorpions and honey bee venoms) for antimicrobial properties against two strains of Gram-negative bacteria Burkholderia pseudomallei by using disc-diffusion assay for in vitro susceptibility testing. The antibacterial activities of the venoms were compared with that of the isolated L-amino acid oxidase (LAAO) and phospholipase A2 (PLA2s) enzymes. MICs were determined using broth dilution method. Bacterial growth was assessed by measurement of optical density at the lowest dilutions (MIC 0.25 mg/ml). The cell viability was measured using tetrazolium salts (XTT) based cytotoxic assay. Results: The studied venoms showed high antimicrobial activity. The venoms of C. adamanteus, Daboia russelli russelli, A. halys, P. australis, B. candidus and P. guttata were equally as effective as Chloramphenicol and Ceftazidime (30 μg/disc). Among those tested, phospholipase A2 enzymes (crotoxin B and daboiatoxin) showed the most potent antibacterial activity against Gram-negative (TES) bacteria. Naturally occurring venom peptides and phospholipase A2 proved to possess highly potent antimicrobial activity against Burkholderia pseudomallei. The XTT-assay results showed that the cell survival decreased with increasing concentrations (0.05-10 mg/mL) of Crotalus adamanteus venom, with no effect on the cell viability evident at 0.5 mg/mL. Conclusion: This antibacterial profile of snake venoms reported herein will be useful in the search for potential antibacterial agents against drug resistant microorganisms like B. pseudomallei.",
author = "{Perumal Samy}, R. and Pachiappan Arjunan and P. Gopalakrishnakone and Thwin, {Maung M.} and Hian, {Yap E.} and Chow, {Vincent T.K.} and Ho Bow and Weng, {Joseph T.}",
year = "2006",
month = "6",
day = "20",
doi = "10.1186/1471-2334-6-100",
language = "English (US)",
volume = "6",
journal = "BMC Infectious Diseases",
issn = "1471-2334",
publisher = "BioMed Central",

}

TY - JOUR

T1 - In vitro antimicrobial activity of natural toxins and animal venoms tested against Burkholderia pseudomallei

AU - Perumal Samy, R.

AU - Arjunan, Pachiappan

AU - Gopalakrishnakone, P.

AU - Thwin, Maung M.

AU - Hian, Yap E.

AU - Chow, Vincent T.K.

AU - Bow, Ho

AU - Weng, Joseph T.

PY - 2006/6/20

Y1 - 2006/6/20

N2 - Background: Burkholderia pseudomallei are the causative agent of melioidosis. Increasing resistance of the disease to antibiotics is a severe problem in treatment regime and has led to intensification of the search for new drugs. Antimicrobial peptides are the most ubiquitous in nature as part of the innate immune system and host defense mechanism. Methods: Here, we investigated a group of venoms (snakes, scorpions and honey bee venoms) for antimicrobial properties against two strains of Gram-negative bacteria Burkholderia pseudomallei by using disc-diffusion assay for in vitro susceptibility testing. The antibacterial activities of the venoms were compared with that of the isolated L-amino acid oxidase (LAAO) and phospholipase A2 (PLA2s) enzymes. MICs were determined using broth dilution method. Bacterial growth was assessed by measurement of optical density at the lowest dilutions (MIC 0.25 mg/ml). The cell viability was measured using tetrazolium salts (XTT) based cytotoxic assay. Results: The studied venoms showed high antimicrobial activity. The venoms of C. adamanteus, Daboia russelli russelli, A. halys, P. australis, B. candidus and P. guttata were equally as effective as Chloramphenicol and Ceftazidime (30 μg/disc). Among those tested, phospholipase A2 enzymes (crotoxin B and daboiatoxin) showed the most potent antibacterial activity against Gram-negative (TES) bacteria. Naturally occurring venom peptides and phospholipase A2 proved to possess highly potent antimicrobial activity against Burkholderia pseudomallei. The XTT-assay results showed that the cell survival decreased with increasing concentrations (0.05-10 mg/mL) of Crotalus adamanteus venom, with no effect on the cell viability evident at 0.5 mg/mL. Conclusion: This antibacterial profile of snake venoms reported herein will be useful in the search for potential antibacterial agents against drug resistant microorganisms like B. pseudomallei.

AB - Background: Burkholderia pseudomallei are the causative agent of melioidosis. Increasing resistance of the disease to antibiotics is a severe problem in treatment regime and has led to intensification of the search for new drugs. Antimicrobial peptides are the most ubiquitous in nature as part of the innate immune system and host defense mechanism. Methods: Here, we investigated a group of venoms (snakes, scorpions and honey bee venoms) for antimicrobial properties against two strains of Gram-negative bacteria Burkholderia pseudomallei by using disc-diffusion assay for in vitro susceptibility testing. The antibacterial activities of the venoms were compared with that of the isolated L-amino acid oxidase (LAAO) and phospholipase A2 (PLA2s) enzymes. MICs were determined using broth dilution method. Bacterial growth was assessed by measurement of optical density at the lowest dilutions (MIC 0.25 mg/ml). The cell viability was measured using tetrazolium salts (XTT) based cytotoxic assay. Results: The studied venoms showed high antimicrobial activity. The venoms of C. adamanteus, Daboia russelli russelli, A. halys, P. australis, B. candidus and P. guttata were equally as effective as Chloramphenicol and Ceftazidime (30 μg/disc). Among those tested, phospholipase A2 enzymes (crotoxin B and daboiatoxin) showed the most potent antibacterial activity against Gram-negative (TES) bacteria. Naturally occurring venom peptides and phospholipase A2 proved to possess highly potent antimicrobial activity against Burkholderia pseudomallei. The XTT-assay results showed that the cell survival decreased with increasing concentrations (0.05-10 mg/mL) of Crotalus adamanteus venom, with no effect on the cell viability evident at 0.5 mg/mL. Conclusion: This antibacterial profile of snake venoms reported herein will be useful in the search for potential antibacterial agents against drug resistant microorganisms like B. pseudomallei.

UR - http://www.scopus.com/inward/record.url?scp=33748889901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748889901&partnerID=8YFLogxK

U2 - 10.1186/1471-2334-6-100

DO - 10.1186/1471-2334-6-100

M3 - Article

C2 - 16784542

AN - SCOPUS:33748889901

VL - 6

JO - BMC Infectious Diseases

JF - BMC Infectious Diseases

SN - 1471-2334

M1 - 100

ER -