In vitro biosynthesis of the tetrasialoganglioside GQ1b.

H. C. Yohe, L. J. Macala, Robert K Yu

Research output: Contribution to journalArticle

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Abstract

A sialyltransferase activity which catalyzes the synthesis of the tetrasialoganglioside GQ1b (N-acetylneuraminyl-N-acetylneuraminylgalactosyl-N-acetylgalactosaminyl [N-acetylneuraminyl-N-acetylneuraminyl]-galactosylglucosylceramide) from added trisialoganglioside GT1b (N-=acetylneuraminylgalactosyl-N-acetylgalactosaminyl [N-acetylneuraminyl-N-acetylneuraminyl]galactosylglucosylceramide) and CMP-N-acetyl[4-14C]neuraminic acid has been demonstrated using a membrane fraction of embryonic chick brain. Optimum enzymatic activity was obtained using the detergent Triton CF-54 at a pH of 6.6. Enzyme activity appeared unaffected by Ca2+, Mg2+, Mn2+, EDTA, or histone. A slight elevation in activity was seen in the presence of Hg2+. When the disialoganglioside GD1b (galactosyl-N-acetylgalactosaminyl [N-acetylneuraminyl-N-acetylneuraminyl]galactosylglucosylceramide) was used as the glycolipid substrate, approximately 15% of the radioactive label was found in GQ1b. When this GQ1b was subjected to a periodate oxidation-borohydride reduction, the distribution of radioactive label was consistent with GQ1b being the major tetrasialoganglioside product and that its synthesis could proceed via the sequence GD1b-GT1b-GQ1b.

Original languageEnglish (US)
Pages (from-to)249-252
Number of pages4
JournalJournal of Biological Chemistry
Volume257
Issue number1
StatePublished - Jan 10 1982
Externally publishedYes

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Neuraminic Acids
Sialyltransferases
Cytidine Monophosphate
Borohydrides
Glycolipids
Biosynthesis
Edetic Acid
Detergents
Histones
Labels
Membranes
Brain
Enzyme activity
Enzymes
Oxidation
Substrates
tetrasialoganglioside GQ1b
sialogangliosides
trisialoganglioside GT1
triton CF 54

ASJC Scopus subject areas

  • Biochemistry

Cite this

In vitro biosynthesis of the tetrasialoganglioside GQ1b. / Yohe, H. C.; Macala, L. J.; Yu, Robert K.

In: Journal of Biological Chemistry, Vol. 257, No. 1, 10.01.1982, p. 249-252.

Research output: Contribution to journalArticle

Yohe, H. C. ; Macala, L. J. ; Yu, Robert K. / In vitro biosynthesis of the tetrasialoganglioside GQ1b. In: Journal of Biological Chemistry. 1982 ; Vol. 257, No. 1. pp. 249-252.
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